快速进展型饮食诱导非酒精性脂肪性肝病动物模型的生物信息学分析。
Bioinformatics analysis of an animal model of diet-induced nonalcoholic fatty liver disease with rapid progression.
机构信息
The Second Central Laboratory, the First Affiliated Hospital of Zhejiang Chinese Medical University, Hangzhou 310016, China.
Key Laboratory of Integrative Chinese and Western Medicine for the Diagnosis and Treatment of Circulatory Diseases of Zhejiang Province, Hangzhou 310016, China.
出版信息
Exp Biol Med (Maywood). 2022 Feb;247(3):263-275. doi: 10.1177/15353702211055099. Epub 2021 Nov 13.
Nonalcoholic fatty liver disease (NAFLD) develops rapidly in high-fat diet (HFD) fed Mongolian gerbil (). Here, we aim to explore the gene expression characteristics of Mongolian gerbil to better understand the underlying mechanism in this animal model. Mongolian gerbils were fed with normal diet or HFD for different periods. High-throughput sequencing was carried out on the hepatic mRNA and bioinformatics analysis was further performed. Eight hub genes Cd44, App, Cdc42, Cd68, Cxcr4, Csf1r, Adgre1, and Fermt3, which were involved in inflammation, fibrosis, and HCC were obtained. Four significant independent poor prognostic factors for HCC (GPC1, ARPC1B, DAB2, and CFL1) were screened out. qRT-PCR result showed that the above genes expressed high levels in different periods of modeling process. The findings of this study provide useful information for further studies on Mongolian gerbil NAFLD model.
非酒精性脂肪性肝病（NAFLD）在高脂肪饮食（HFD）喂养的蒙古沙鼠中迅速发展（）。在这里，我们旨在探索蒙古沙鼠的基因表达特征，以更好地了解该动物模型中的潜在机制。蒙古沙鼠分别用正常饮食或 HFD 喂养不同时间。对肝 mRNA 进行高通量测序，并进一步进行生物信息学分析。获得了涉及炎症、纤维化和 HCC 的 8 个关键基因 Cd44、App、Cdc42、Cd68、Cxcr4、Csf1r、Adgre1 和 Fermt3。筛选出 4 个 HCC 的显著独立预后不良因素（GPC1、ARPC1B、DAB2 和 CFL1）。qRT-PCR 结果显示，上述基因在建模过程的不同时期表达水平较高。本研究的结果为进一步研究蒙古沙鼠 NAFLD 模型提供了有用的信息。
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