Barczynski J L, Fletcher R D, Segal A H, Conway J C
Department of Periodontics, University of Pittsburgh School of Dental Medicine, PA 15261.
J Periodontol. 1987 Sep;58(9):622-7. doi: 10.1902/jop.1987.58.9.622.
Interest has recently been directed towards the use of antiplaque mouthrinses. Most published material concerns the antimicrobial effects of these agents rather than their effects upon oral tissue. This study was conducted to evaluate the effect of a sanguinarine-containing mouthrinse called Viadent upon epithelial-like gingival cells. The cells were grown for 24 hours in supplemented Earle's medium, with and without different Viadent dilutions. Cell counts were made with a hematocytometer. It was found that 50% of the cells were inhibited at 1.2% Viadent. In similar studies, it was found that 70% ethanol and two pH buffers were less toxic than Viadent. Exposure of preformed cell monolayers to Viadent also showed significant inhibition. The relative toxicity of different antiplaque agents may be compared using such cells as a model system. In conclusion, it was observed that Viadent significantly affected gingival cell growth in vitro, that viable cell numbers were greatly reduced by short time exposure, and that the toxic effect of Viadent could only partially be accounted for by ethanol content and/or pH.
近来，人们的兴趣已转向使用抗牙菌斑漱口水。大多数已发表的材料关注的是这些药剂的抗菌作用，而非它们对口腔组织的影响。本研究旨在评估一种名为Viadent的含血根碱漱口水对上皮样牙龈细胞的影响。将细胞在添加了Earle培养基的条件下培养24小时，分别添加和不添加不同稀释度的Viadent。用血细胞计数器进行细胞计数。结果发现，当Viadent浓度为1.2%时，50%的细胞受到抑制。在类似研究中，发现70%乙醇和两种pH缓冲液的毒性低于Viadent。将预先形成的细胞单层暴露于Viadent也显示出显著抑制作用。可以使用此类细胞作为模型系统来比较不同抗牙菌斑药剂的相对毒性。总之，观察到Viadent在体外显著影响牙龈细胞生长，短时间暴露会使活细胞数量大幅减少，且Viadent的毒性作用只能部分由乙醇含量和/或pH来解释。
