State Key Laboratory of Food Science and Technology, Nanchang University, Nanchang, Jiangxi 330047, China.
State Key Laboratory of Food Science and Technology, Nanchang University, Nanchang, Jiangxi 330047, China.
Food Chem. 2022 Mar 30;373(Pt B):131539. doi: 10.1016/j.foodchem.2021.131539. Epub 2021 Nov 8.
The protective effect of antioxidant peptides from grass carp scale gelatin on hydrogen peroxide (HO)-mediated oxidative injured HepG2 cells was investigated, and the protective mechanism as well as peptide structure were studied. Pretreated with grass carp scale gelatin hydrolysates (GSGH) for 24 h significantly increased the survival rates and decreased the apoptosis rates of HO-mediated oxidative injured HepG2 cells. The increase in SOD, CAT and GPX activities, reduce in ROS level and MDA content, and weaken in damage on cell membrane and DNA could be responsible for the protective effect of GSGH on HO-mediated oxidative injured HepG2 cells. Peptide sequences of GSGH were analyzed by LC-ESI-Q-Orbitrap-MS/MS, and results showed that most of them were low molecular weight peptide at 358-986 Da. Synergistic effect existed among antioxidant peptides and contributed to the strong antioxidant activities of GSGH.
草鱼鱼鳞明胶抗氧化肽对过氧化氢（HO）介导的氧化损伤 HepG2 细胞的保护作用进行了研究，并对其保护机制和肽结构进行了研究。用草鱼鱼鳞明胶水解物（GSGH）预处理 24 h 可显著提高 HO 介导的氧化损伤 HepG2 细胞的存活率，降低细胞凋亡率。GSGH 对 HO 介导的氧化损伤 HepG2 细胞的保护作用可能与 SOD、CAT 和 GPX 活性的增加、ROS 水平和 MDA 含量的降低、细胞膜和 DNA 损伤的减弱有关。通过 LC-ESI-Q-Orbitrap-MS/MS 对 GSGH 的肽序列进行分析，结果表明，大多数肽的分子量为 358-986 Da。抗氧化肽之间存在协同作用，这也是 GSGH 具有较强抗氧化活性的原因。
