Key Laboratory of Biorheological Science and Technology, Ministry of Education, Bioengineering College, Chongqing University, Chongqing 400044, PR China.
Chongqing Academy of Animal Science, Chongqing Engineering Research Center of Meat Quality Evaluation and Processing, Rongchang, Chongqing 402460, PR China.
Poult Sci. 2019 Dec 1;98(12):7118-7128. doi: 10.3382/ps/pez430.
Previous work showed that peptides from duck eggs incubated for 15 D presented high total antioxidant activities. Here, this work explore the antioxidant activities of different segments, ZT1 (≤3 KD), ZT2 (≤10 KD), and ZT3 (≤30 KD), derived from duck embryo peptides and their protective effects against H2O2-induced oxidative damage in HepG2 cells. Peptides present no cytotoxicity to HepG2 cells. Moreover, ZT1 exhibits a higher ability to scavenge several radicals as well as stronger inhibition of H2O2-induced oxidative stress than ZT2 and ZT3. The activities of catalase and glutathione peroxidase as well as total superoxide dismutase increase in a concentration-dependent manner. Peptides are isolated from ZT1 and then subjected to LC-MS/MS to identify their sequences, followed by functional annotation, bioinformatics prediction, and hot-spot motif recognition. As a result, 413 potential functional peptides are identified, with some compounds exhibiting more than 1 function. This work will help for exploring bioactive substances in duck embryo eggs and enhance the utilization value of duck or other poultry eggs.
先前的研究表明，孵化 15 天的鸭蛋肽具有较高的总抗氧化活性。本研究探索了来自鸭胚胎肽的不同片段 ZT1（≤3 KD）、ZT2（≤10 KD）和 ZT3（≤30 KD）的抗氧化活性及其对 HepG2 细胞中 H2O2 诱导的氧化损伤的保护作用。肽对 HepG2 细胞没有细胞毒性。此外，ZT1 清除几种自由基的能力以及抑制 H2O2 诱导的氧化应激的能力均强于 ZT2 和 ZT3。过氧化氢酶、谷胱甘肽过氧化物酶和总超氧化物歧化酶的活性呈浓度依赖性增加。肽从 ZT1 中分离出来，然后进行 LC-MS/MS 鉴定其序列，随后进行功能注释、生物信息学预测和热点基序识别。结果鉴定出 413 个潜在的功能肽，其中一些化合物具有超过 1 种功能。这项工作将有助于探索鸭胚胎蛋中的生物活性物质，并提高鸭或其他家禽蛋的利用价值。
