van het Schip F D, Samallo J, Broos J, Ophuis J, Mojet M, Gruber M, AB G
Department of Biochemistry, University of Groningen, The Netherlands.
J Mol Biol. 1987 Jul 20;196(2):245-60. doi: 10.1016/0022-2836(87)90688-7.
The gene encoding the major vitellogenin from chicken has been completely sequenced and its exon-intron organization has been established. The gene is 20,342 base-pairs long and contains 35 exons with a combined length of 5787 base-pairs. They encode the 1850-amino acid pre-peptide of vitellogenin, which is the precursor of the mature yolk proteins, the serine-rich and heavily phosphorylated phosvitin and the lipovitellin. The 217-amino acid phosvitin polypeptide occupies an internal position (residue 1112 through 1328) within the vitellogenin molecule. The 125,000 and 30,000 Mr lipovitellin polypeptides are encoded by the sequences at the N-terminal and the C-terminal sides of the phosvitin section, respectively. The main features of the gene and protein sequences, and the evolutionary implications, are discussed.
编码鸡主要卵黄蛋白原的基因已被完全测序，其外显子-内含子结构也已确定。该基因长度为20342个碱基对，包含35个外显子，总长度为5787个碱基对。它们编码1850个氨基酸的卵黄蛋白原前体肽，该前体肽是成熟卵黄蛋白、富含丝氨酸且高度磷酸化的卵黄高磷蛋白和卵黄脂磷蛋白的前体。217个氨基酸的卵黄高磷蛋白多肽位于卵黄蛋白原分子内部位置（第1112至1328位残基）。125000和30000道尔顿的卵黄脂磷蛋白多肽分别由卵黄高磷蛋白部分N端和C端的序列编码。文中讨论了该基因和蛋白质序列的主要特征及其进化意义。
