Byrne B M, van het Schip A D, van de Klundert J A, Arnberg A C, Gruber M, Ab G
Biochemistry. 1984 Sep 11;23(19):4275-9. doi: 10.1021/bi00314a003.
The amino acid sequence of the egg yolk storage protein phosvitin has been deduced from the nucleotide sequence of part of the chicken vitellogenin gene. Of the phosvitin sequence, 210 amino acids including the N-terminal residue are contained on one large exon, whereas the remaining six amino acids are encoded on the next exon. Phosvitin contains a core region of 99 amino acids, consisting of 80 serines, grouped in runs of maximally 14 residues interspersed by arginines, lysines, and asparagines. The serines of the core region are encoded by AGC and AGT codons exclusively and the arginines by AGA and AGG, which results in a continuous stretch of 99 codons with adenine in the first position. The N-terminal quarter of the phosvitin sequence contains 16 serines grouped in a cluster with alanines and threonines and coded mainly by TCX triplets. The C-terminal part includes 27 serines, preferentially coded by AGC and AGT, 13 histidine residues, and the sequence ...Asn-Gly-Ser... at which the carbohydrate moiety of phosvitin is attached. Heteroduplex formation between cloned DNAs from chicken and Xenopus vitellogenin genes shows that the phosvitin sequence contains a stretch of highly conserved sequence.
卵黄储存蛋白卵黄高磷蛋白的氨基酸序列已从鸡卵黄生成素基因部分的核苷酸序列推导得出。在卵黄高磷蛋白序列中，包括N端残基在内的210个氨基酸包含在一个大外显子上，而其余6个氨基酸则由下一个外显子编码。卵黄高磷蛋白包含一个由99个氨基酸组成的核心区域，其中有80个丝氨酸，以最长14个残基的片段形式排列，中间穿插着精氨酸、赖氨酸和天冬酰胺。核心区域的丝氨酸仅由AGC和AGT密码子编码，精氨酸由AGA和AGG编码，这导致了一段连续的99个密码子，第一位都是腺嘌呤。卵黄高磷蛋白序列的N端四分之一包含16个丝氨酸，与丙氨酸和苏氨酸聚集成簇，主要由TCX三联体编码。C端部分包括27个丝氨酸（优先由AGC和AGT编码）、13个组氨酸残基，以及卵黄高磷蛋白碳水化合物部分所连接的序列……天冬酰胺-甘氨酸-丝氨酸……。鸡和非洲爪蟾卵黄生成素基因克隆DNA之间的异源双链形成表明，卵黄高磷蛋白序列包含一段高度保守的序列。
