Yamaguchi Naoya, Taguchi-Shiobara Fumio, Sato Yumi, Senda Mineo, Ishimoto Masao, Kousaka Fumiko
Hokkaido Research Organization Tokachi Agricultural Experiment Station, 2, Minami 9 sen, Shinsei, Memuro-cho, Kasai-gun, Hokkaido 082-0081, Japan.
National Institute of Crop Science, National Agriculture and Food Research Organization, Kannondai, Tsukuba, Ibaraki 305-8518, Japan.
Breed Sci. 2021 Jun;71(3):396-403. doi: 10.1270/jsbbs.20153. Epub 2021 Jun 19.
In soybean [ (L.) Merrill], the genetic analysis of seed yield is important to aid in the breeding of high-yielding cultivars. Seed yield is a complex trait, and the number of quantitative trait loci (QTL) involved in seed yield is high. The aims of this study were to identify QTL associated with seed yield and validate their effects on seed yield using near-isogenic lines. The QTL analysis was conducted using a recombinant inbred line population derived from a cross between Japanese cultivars 'Toyoharuka' and 'Toyomusume', and eight seed yield-associated QTL were identified. There were significant positive correlations between seed yield and the number of favorable alleles at QTL associated with seed yield in the recombinant inbred lines for three years. The effects of , a QTL promoting greater seed yield, was validated in the Toyoharuka background. In a two-year yield trial, the 100-seed weight and seed yield of Toyoharuka-NIL, the near-isogenic line having the Toyomusume allele at , were significantly greater than those of Toyoharuka (106% and 107%, respectively) without any change for days to flowering and maturity. Our results suggest that was not associated with maturity genes, and contributed to the 100-seed weight.
在大豆[（L.）梅里尔]中，种子产量的遗传分析对于高产栽培品种的育种具有重要意义。种子产量是一个复杂的性状，涉及种子产量的数量性状位点（QTL）数量众多。本研究的目的是鉴定与种子产量相关的QTL，并利用近等基因系验证它们对种子产量的影响。使用源自日本品种“丰春香”和“丰梦姬”杂交的重组自交系群体进行QTL分析，鉴定出8个与种子产量相关的QTL。在连续三年的重组自交系中，种子产量与种子产量相关QTL上有利等位基因的数量之间存在显著正相关。在“丰春香”背景下验证了一个促进种子产量提高的QTL的效应。在为期两年的产量试验中，在该QTL上具有“丰梦姬”等位基因的近等基因系“丰春香-NIL”的百粒重和种子产量显著高于“丰春香”（分别为106%和107%），而开花天数和成熟天数没有任何变化。我们的结果表明，该QTL与成熟基因无关，且对百粒重有贡献。
