Palomeque Laura, Li-Jun Liu, Li Wenbin, Hedges Bradley, Cober Elroy R, Rajcan Istvan
Department of Plant Agriculture, Crop Science Building, University of Guelph, Guelph, ON N1G 2W1, Canada.
Theor Appl Genet. 2009 Aug;119(3):429-36. doi: 10.1007/s00122-009-1048-8. Epub 2009 May 22.
Seed yield mega-environment-universal and specific QTL (QTL(U) and QTL(SP), respectively) linked to Satt100, Satt130, Satt162, Satt194, Satt259 Satt277 and Sat_126, have been identified in a population derived from a cross between a Chinese and a Canadian soybean [Glycine max (L.) Merrill] elite line. The variation observed in yield could be the consequence of the variation of agronomic traits. Yield-component traits have been reported in the literature, but a better understanding of their impact at the molecular level is still lacking. Therefore, the objectives of this study were to identify traits correlated with yield and to determine if the yield QTL(U) and QTL(SP) were co-localized with QTL(U) and QTL(SP) associated with an agronomic trait. A recombinant inbred line (RIL) population was developed from a cross between a high-yielding adapted Canadian and a high-yielding exotic Chinese soybean elite line. The RIL were evaluated in multiple environments in China and Canada during the period from 2004 to 2006. Four yield QTL(U,) tagged by markers Satt100, Satt277, Satt162 and Sat_126, were co-localized with a QTL associated with an agronomic trait, behaving as either QTL(U) or QTL(SP) for the agronomic trait. For example, the yield QTL(U,) tagged by marker Satt100 was associated also with 100 seed weight, pods per plant, pods per node, plant height, R1, R5, R8, oil content and protein content in all Canadian environments, but only with pods per plant, pods per node, plant height, R1, R5, R8 and oil content in two or more Chinese environments. No agronomic traits QTL were co-localized with the yield QTL(U) tagged by the marker Satt139 or the yield QTL(SP) tagged by Satt259, suggesting a physiological basis of the yield in these QTL. The results suggest that a successful introgression of crop productivity alleles from plant introductions into an adapted germplasm could be facilitated by the use of both the QTL(U) and QTL(SP) because each type of QTL contributed either directly or indirectly through yield-component traits to seed yield of RILs.
在一个由中国和加拿大大豆[Glycine max (L.) Merrill]优良品系杂交产生的群体中，已鉴定出与Satt100、Satt130、Satt162、Satt194、Satt259、Satt277和Sat_126连锁的种子产量大环境通用和特定QTL（分别为QTL(U)和QTL(SP)）。观察到的产量变异可能是农艺性状变异的结果。文献中已报道了产量构成性状，但在分子水平上对其影响仍缺乏更深入的了解。因此，本研究的目的是确定与产量相关的性状，并确定产量QTL(U)和QTL(SP)是否与与农艺性状相关的QTL(U)和QTL(SP)共定位。一个重组自交系（RIL）群体由一个高产适应性加拿大大豆和一个高产外来中国大豆优良品系杂交产生。在2004年至2006年期间，在中国和加拿大的多个环境中对RIL进行了评估。由标记Satt100、Satt277、Satt162和Sat_126标记的四个产量QTL(U)与一个与农艺性状相关的QTL共定位，对该农艺性状表现为QTL(U)或QTL(SP)。例如，由标记Satt100标记的产量QTL(U)在所有加拿大环境中还与百粒重、单株荚数、每节荚数、株高、R1、R5、R8、油含量和蛋白质含量相关，但在两个或更多中国环境中仅与单株荚数、每节荚数、株高、R1、R5、R8和油含量相关。没有农艺性状QTL与由标记Satt139标记的产量QTL(U)或由Satt^259标记的产量QTL(SP)共定位，这表明这些QTL中产量的生理基础。结果表明，通过使用QTL(U)和QTL(SP)，可以促进将植物引进材料中的作物生产力等位基因成功导入适应性种质，因为每种类型的QTL都直接或间接地通过产量构成性状对RIL的种子产量有贡献。
