Wang Xicheng, Yan Lichun, Wang Bo, Qian Yaming, Wang Zhuangwei, Wu Weimin
Institute of Pomology, Jiangsu Academy of Agricultural Sciences, Jiangsu Key Laboratory for Horticultural Crop Genetic Improvement, Nanjing, China.
Front Plant Sci. 2021 Oct 29;12:749184. doi: 10.3389/fpls.2021.749184. eCollection 2021.
Waterlogging severely affects global agricultural production. Clarifying the regulatory mechanism of grapevine in response to waterlogging stress will help to improve the waterlogging tolerance of grapevine. In the present study, the physiological and proteomic responses of SO4 grapevine rootstock to different waterlogging tolerances were comparatively assayed. The results showed that the activities of SOD and POD first increased and then decreased, while the change trend of CAT and APX activities was the opposite. In addition, the MDA and HO contents increased after waterlogging treatment, but the chlorophyll a and chlorophyll b contents decreased. A total of 5,578 grapevine proteins were identified by the use of the tandem mass tag (TMT) labeling technique. Among them, 214 (103 and 111 whose expression was upregulated and downregulated, respectively), 314 (129 and 185 whose expression was upregulated and downregulated, respectively), and 529 (248 and 281 whose expression was upregulated and downregulated, respectively) differentially expressed proteins (DEPs) were identified in T0d vs. T10d, T10d vs. T20d, and T0d vs. T20d comparison groups, respectively. Enrichment analysis showed that these DEPs were mainly involved in glutathione metabolism, carbon fixation, amino sugar and nucleotide sugar metabolism, biosynthesis of amino acids, photosynthesis, carbon metabolism, starch, and sucrose metabolism, galactose metabolism, protein processing and ribosomes. To further verify the proteomic data, the expression of corresponding genes that encode eight DEPs was confirmed by quantitative reverse transcriptase-polymerase chain reaction (qRT-PCR). The results of this study presented an important step toward understanding the resistance mechanisms of grapevine in response to waterlogging stress at the proteome level.
涝害严重影响全球农业生产。阐明葡萄对涝害胁迫的调控机制将有助于提高葡萄的耐涝性。在本研究中，对SO4葡萄砧木对不同耐涝性的生理和蛋白质组学反应进行了比较分析。结果表明，超氧化物歧化酶（SOD）和过氧化物酶（POD）的活性先升高后降低，而过氧化氢酶（CAT）和抗坏血酸过氧化物酶（APX）活性的变化趋势则相反。此外，涝害处理后丙二醛（MDA）和过氧化氢（HO）含量增加，但叶绿素a和叶绿素b含量降低。使用串联质谱标签（TMT）标记技术共鉴定出5578种葡萄蛋白。其中，在T0d与T10d、T10d与T20d、T0d与T20d比较组中分别鉴定出214种（分别有103种和111种表达上调和下调）、314种（分别有129种和185种表达上调和下调）和529种（分别有248种和281种表达上调和下调）差异表达蛋白（DEP）。富集分析表明，这些DEP主要参与谷胱甘肽代谢、碳固定、氨基糖和核苷酸糖代谢、氨基酸生物合成、光合作用、碳代谢、淀粉和蔗糖代谢、半乳糖代谢、蛋白质加工和核糖体。为进一步验证蛋白质组学数据，通过定量逆转录聚合酶链反应（qRT-PCR）确认了编码8种DEP的相应基因的表达。本研究结果为在蛋白质组水平上理解葡萄对涝害胁迫的抗性机制迈出了重要一步。
