Liu Guo-Tian, Wang Bian-Bian, Lecourieux David, Li Mei-Jie, Liu Ming-Bo, Liu Rui-Qi, Shang Bo-Xing, Yin Xiao, Wang Li-Jun, Lecourieux Fatma, Xu Yan
State Key Laboratory of Crop Stress Biology for Arid Areas, College of Horticulture, Northwest A&F University, Yangling, China.
Key Laboratory of Horticultural Plant Biology and Germplasm Innovation in Northwest China, Ministry of Agriculture, Northwest A&F University, Yangling, China.
Hortic Res. 2021 May 1;8(1):100. doi: 10.1038/s41438-021-00533-y.
Wild grapevines can show strong resistance to the downy mildew pathogen P. viticola, but the associated mechanisms are poorly described, especially at early stages of infection. Here, we performed comparative proteomic analyses of grapevine leaves from the resistant genotype V. davidii "LiuBa-8" (LB) and susceptible V. vinifera "Pinot Noir" (PN) 12 h after inoculation with P. viticola. By employing the iTRAQ technique, a total of 444 and 349 differentially expressed proteins (DEPs) were identified in LB and PN, respectively. The majority of these DEPs were related to photosynthesis, respiration, cell wall modification, protein metabolism, stress, and redox homeostasis. Compared with PN, LB showed fewer downregulated proteins associated with photosynthesis and more upregulated proteins associated with metabolism. At least a subset of PR proteins (PR10.2 and PR10.3) was upregulated upon inoculation in both genotypes, whereas HSP (HSP70.2 and HSP90.6) and cell wall-related XTH and BXL1 proteins were specifically upregulated in LB and PN, respectively. In the incompatible interaction, ROS signaling was evident by the accumulation of HO, and multiple APX and GST proteins were upregulated. These DEPs may play crucial roles in the grapevine response to downy mildew. Our results provide new insights into molecular events associated with downy mildew resistance in grapevine, which may be exploited to develop novel protection strategies against this disease.
野生葡萄藤对霜霉病病原菌葡萄生单轴霉表现出很强的抗性，但其相关机制的描述却很少，尤其是在感染的早期阶段。在此，我们对接种葡萄生单轴霉12小时后的抗性基因型刺葡萄“刘八-8”（LB）和感病的酿酒葡萄“黑比诺”（PN）的葡萄叶片进行了比较蛋白质组学分析。通过采用iTRAQ技术，在LB和PN中分别鉴定出444个和349个差异表达蛋白（DEP）。这些DEP中的大多数与光合作用、呼吸作用、细胞壁修饰、蛋白质代谢、胁迫和氧化还原稳态有关。与PN相比，LB中与光合作用相关的下调蛋白较少，与代谢相关的上调蛋白较多。在两种基因型中，接种后至少一部分病程相关蛋白（PR10.2和PR10.3）上调，而热激蛋白（HSP70.2和HSP90.6）以及与细胞壁相关的木葡聚糖内转糖基酶和β-木糖苷酶1蛋白分别在LB和PN中特异性上调。在不亲和互作中，通过过氧化氢的积累可明显看出活性氧信号，并且多种抗坏血酸过氧化物酶和谷胱甘肽S-转移酶蛋白上调。这些DEP可能在葡萄对霜霉病的反应中起关键作用。我们的结果为与葡萄霜霉病抗性相关的分子事件提供了新的见解，这可能有助于开发针对这种病害的新型保护策略。
