Ouyang Chao, Liu Wei, Chen Silan, Zhao Huimin, Chen Xinyan, Jin Xiongxia, Li Xinpeng, Wu Yongzhong, Zeng Xiang, Huang Peijin, He Xiuying, An Baoguang
Hainan Bolian Rice Gene Technology Co., Ltd., Haikou, China.
Guangdong Provincial Key Laboratory of New Technology in Rice Breeding, Guangzhou, China.
Front Plant Sci. 2021 Oct 29;12:756116. doi: 10.3389/fpls.2021.756116. eCollection 2021.
Glyphosate-resistant crops developed by the gene from have been planted on a massive scale globally, which benefits from the high efficiency and broad spectrum of glyphosate in weed control. Some glyphosate-resistant (GR) genes from microbes have been reported, which might raise biosafety concerns. Most of them were obtained through a hygromycin- transformation system. Here we reported the plant source with 5-enolpyruvylshikimate-3-phosphate synthase () gene from goosegrass endowed rice with high resistance to glyphosate. The integrations and inheritability of the transgenes in the rice genome were investigated within two generations. The transgenic plants displayed similar growth and development to wild type under no glyphosate selection pressure but better reproductive performance under lower glyphosate selection pressure. Furthermore, we reconstructed a binary vector pCEiEPSPS and established the whole stage glyphosate selection using the vector. The Glyphosate-pCEiEPSPS selection system showed a significantly higher transformation efficiency compared with the hygromycin- transformation system. Our results provided a promising alternative gene resource to the development of GR plants and also extended the plant transformation toolbox.
通过来自[未提及具体来源]的基因培育出的抗草甘膦作物已在全球大规模种植，这得益于草甘膦在杂草控制方面的高效性和广谱性。一些来自微生物的抗草甘膦（GR）基因已被报道，这可能引发生物安全问题。其中大多数是通过潮霉素转化系统获得的。在此，我们报道了一种植物来源，其具有来自稗草的5-烯醇丙酮酰莽草酸-3-磷酸合酶（EPSPS）基因，该基因赋予水稻对草甘膦的高抗性。在两代内研究了转基因在水稻基因组中的整合情况和遗传性。在无草甘膦选择压力下，EPSPS转基因植株的生长发育与野生型相似，但在较低草甘膦选择压力下具有更好的生殖性能。此外，我们构建了二元载体pCEiEPSPS，并使用该载体建立了全阶段草甘膦选择体系。与潮霉素转化系统相比，草甘膦-pCEiEPSPS选择系统显示出显著更高的转化效率。我们的结果为GR植物的开发提供了一种有前景的替代基因资源，也扩展了植物转化工具箱。
