文献检索文档翻译深度研究
Suppr Zotero 插件Zotero 插件
邀请有礼套餐&价格历史记录

新学期，新优惠

限时优惠:9月1日-9月22日

30天高级会员仅需29元

1天体验卡首发特惠仅需5.99元

了解详情
不再提醒
插件&应用
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
高级版
套餐订阅购买积分包
AI 工具
文献检索文档翻译深度研究
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2025

ANLN 受 miR-30a-5p 调控，介导肺腺癌的恶性进展。

ANLN Regulated by miR-30a-5p Mediates Malignant Progression of Lung Adenocarcinoma.

机构信息

Department of Thoracic Surgery, Jiangyin Hospital of Traditional Chinese Medicine, Jiangyin Hospital Affiliated to Nanjing University of Chinese Medicine, Jiangyin 214400, China.

出版信息

Comput Math Methods Med. 2021 Nov 5;2021:9549287. doi: 10.1155/2021/9549287. eCollection 2021.

DOI:10.1155/2021/9549287
PMID:34777569
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC8589480/
Abstract

BACKGROUND: ANLN and miR-30a-5p may be involved in the progression of lung adenocarcinoma (LUAD). However, their underlying mechanism in LUAD has not been completely comprehended. METHODS: Differential expression analysis, binding site prediction, and survival analysis were conducted by bioinformatics approaches. ANLN mRNA and miR-30a-5p expression were detected by qRT-PCR. ANLN protein expression was detected by western blot. Cell behaviors in LUAD were examined by functional experiments. RESULTS: ANLN was activated in LUAD cells in terms of mRNA and protein. High ANLN level was positively correlated with poor prognosis. Enforced ANLN stimulated protumorigenesis LUAD cell behaviors. miR-30a-5p could target ANLN mRNA, as revealed and verified through assays. Remarkably low miR-30a-5p expression was observed in LUAD cells, and it could repress ANLN expression. The accelerated cell behaviors by overexpression of ANLN were counteracted by upregulating miR-30a-5p. CONCLUSION: Overall, miR-30a-5p remarkably restrained the malignant progression of LUAD cells by constraining ANLN expression. Thus, ANLN and miR-30a-5p could be novel therapeutic targets of LUAD.

摘要

背景：卷曲螺旋结构域蛋白 22（ANLN）和 miR-30a-5p 可能参与肺腺癌（LUAD）的进展。然而，其在 LUAD 中的潜在机制尚未完全阐明。

方法：通过生物信息学方法进行差异表达分析、结合位点预测和生存分析。采用 qRT-PCR 检测 ANLN mRNA 和 miR-30a-5p 的表达。采用 Western blot 检测 ANLN 蛋白表达。通过功能实验检测 LUAD 中的细胞行为。

结果：在 LUAD 细胞中，ANLN 在 mRNA 和蛋白水平上均被激活。高 ANLN 水平与不良预后呈正相关。过表达 ANLN 可刺激促肿瘤发生的 LUAD 细胞行为。miR-30a-5p 可以靶向 ANLN mRNA，通过实验证实和验证。在 LUAD 细胞中观察到 miR-30a-5p 表达显著降低，可抑制 ANLN 表达。过表达 ANLN 加速的细胞行为可通过上调 miR-30a-5p 得到抑制。

结论：总体而言，miR-30a-5p 通过抑制 ANLN 表达显著抑制 LUAD 细胞的恶性进展。因此，ANLN 和 miR-30a-5p 可能成为 LUAD 的新治疗靶点。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/eae3/8589480/1cbedfca7d61/CMMM2021-9549287.005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/eae3/8589480/d92226c552a1/CMMM2021-9549287.001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/eae3/8589480/53c7f6bd5294/CMMM2021-9549287.002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/eae3/8589480/02ebd6d7dc1a/CMMM2021-9549287.003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/eae3/8589480/f4db290728fd/CMMM2021-9549287.004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/eae3/8589480/1cbedfca7d61/CMMM2021-9549287.005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/eae3/8589480/d92226c552a1/CMMM2021-9549287.001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/eae3/8589480/53c7f6bd5294/CMMM2021-9549287.002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/eae3/8589480/02ebd6d7dc1a/CMMM2021-9549287.003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/eae3/8589480/f4db290728fd/CMMM2021-9549287.004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/eae3/8589480/1cbedfca7d61/CMMM2021-9549287.005.jpg

相似文献

[1]
ANLN Regulated by miR-30a-5p Mediates Malignant Progression of Lung Adenocarcinoma.

Comput Math Methods Med. 2021

[2]
miR-30a-5p Regulates Viability, Migration, and Invasion of Lung Adenocarcinoma Cells via Targeting ECT2.

Comput Math Methods Med. 2021

[3]
miR-139-5p Inhibits Lung Adenocarcinoma Cell Proliferation, Migration, and Invasion by Targeting MAD2L1.

Comput Math Methods Med. 2020

[4]
miR-21-5p promotes lung adenocarcinoma cell proliferation, migration and invasion via targeting WWC2.

Cancer Biomark. 2020

[5]
Vascular endothelial cell-derived exosomal miR-30a-5p inhibits lung adenocarcinoma malignant progression by targeting CCNE2.

Carcinogenesis. 2021-8-19

[6]
miR-182-5p Serves as an Oncogene in Lung Adenocarcinoma through Binding to STARD13.

Comput Math Methods Med. 2021

[7]
LINC02535/miR-30a-5p/GALNT3 axis contributes to lung adenocarcinoma progression via the NF- κ B signaling pathway.

Cell Cycle. 2022-12

[8]
MiRNA-30a-5p/VCAN Arrests Tumor Metastasis via Modulating the Adhesion of Lung Adenocarcinoma Cells.

Appl Biochem Biotechnol. 2023-12

[9]
miR-9-5p Promotes Lung Adenocarcinoma Cell Proliferation, Migration and Invasion by Targeting ID4.

Technol Cancer Res Treat. 2021

[10]
MiR-30a-3p Suppresses the Growth and Development of Lung Adenocarcinoma Cells Through Modulating GOLM1/JAK-STAT Signaling.

Mol Biotechnol. 2022-10

引用本文的文献

[1]
Investigating miR-30a's tumor suppressor role and its targets (MYBL2, TWF1, GALNT7, PFN2) as prognostic biomarkers in lung adenocarcinoma.

Biochem Biophys Rep. 2025-7-8

[2]
Development of a reliable risk prognostic model for lung adenocarcinoma based on the genes related to endotheliocyte senescence.

Sci Rep. 2025-4-12

[3]
Therapeutic and prognostic effect of disulfidptosis-related genes in lung adenocarcinoma.

Heliyon. 2024-6-29

[4]
Integrated bulk and single-cell RNA sequencing identifies an aneuploidy-based gene signature to predict sensitivity of lung adenocarcinoma to traditional chemotherapy drugs and patients' prognosis.

PeerJ. 2024

[5]
Hsa_circ_0079875 functions as a competitive endogenous RNA to promote hepatocellular carcinoma progression.

Cell Cycle. 2024-3

[6]
Retracted: ANLN Regulated by miR-30a-5p Mediates Malignant Progression of Lung Adenocarcinoma.

Comput Math Methods Med. 2023-10-4

[7]
Bioinformatics Analysis and Screening of Potential Target Genes Related to the Lung Cancer Prognosis.

Med Princ Pract. 2023-9-14

[8]
Knockdown of ANLN inhibits the progression of lung adenocarcinoma via pyroptosis activation.

Mol Med Rep. 2023-9

[9]
Tumor-suppressive action of miR-30a-5p in lung adenocarcinoma correlates with ABL2 inhibition and PI3K/AKT pathway inactivation.

Clin Transl Oncol. 2024-2

[10]
The mitotic spindle-related seven-gene predicts the prognosis and immune microenvironment of lung adenocarcinoma.

J Cancer Res Clin Oncol. 2023-9

本文引用的文献

[1]
MiR-593-5p inhibited proliferation and migration of lung adenocarcinoma by targeting ICAM-1.

Eur Rev Med Pharmacol Sci. 2020-4

[2]
miR-30a-5p Inhibits Proliferation and Migration of Lung Squamous Cell Carcinoma Cells by Targeting FOXD1.

Biomed Res Int. 2020

[3]
Circular RNA circCRIM1 suppresses lung adenocarcinoma cell migration, invasion, EMT, and glycolysis through regulating miR-125b-5p/BTG2 axis.

Eur Rev Med Pharmacol Sci. 2020-4

[4]
Circulating microRNA/isomiRs as novel biomarkers of esophageal squamous cell carcinoma.

PLoS One. 2020-4-6

[5]
The Lnc LINC00461/miR-30a-5p facilitates progression and malignancy in non-small cell lung cancer via regulating ZEB2.

Cell Cycle. 2020-4

[6]
Identification of key microRNAs and hub genes in non-small-cell lung cancer using integrative bioinformatics and functional analyses.

J Cell Biochem. 2020-3

[7]
Down-regulation of miR-30a-5p is Associated with Poor Prognosis and Promotes Chemoresistance of Gemcitabine in Pancreatic Ductal Adenocarcinoma.

J Cancer. 2019-8-28

[8]
ANLN-induced EZH2 upregulation promotes pancreatic cancer progression by mediating miR-218-5p/LASP1 signaling axis.

J Exp Clin Cancer Res. 2019-8-8

[9]
[Effect of long-chain non-coding RNA-AC024560.2 on proliferation and invasion of prostate cancer cells by targeted regulation of miR-30a-5p].

Zhonghua Yi Xue Za Zhi. 2019-7-9

[10]
The lncRNA FEZF1-AS1 Promotes the Progression of Colorectal Cancer Through Regulating OTX1 and Targeting miR-30a-5p.

Oncol Res. 2019-7-3

文献AI研究员

20分钟写一篇综述，助力文献阅读效率提升50倍

立即体验

用中文搜PubMed

大模型驱动的PubMed中文搜索引擎

马上搜索

推荐工具

医学文档翻译智能文献检索