Nyvad B, Kilian M
Department of Oral Anatomy, Dental Pathology and Operative Dentistry, Royal Dental College, Aarhus, Denmark.
Scand J Dent Res. 1987 Oct;95(5):369-80. doi: 10.1111/j.1600-0722.1987.tb01627.x.
This study describes the predominant early microflora on human teeth on the basis of microbiologic identification of 1742 fresh isolates. The isolates were obtained from four dental students who carried test pieces of enamel and root surface in the oral cavity for 4, 8, 12, and 24 h. During the experimental periods oral hygiene was discontinued. Under equal conditions root surfaces were more heavily colonized than were enamel surfaces. However, the composition of the microbiota was the same. Within the first 24 h the microflora was dominated by streptococci and Gram-positive pleomorphic rods. S. sanguis contributed only 6-18% of the early colonizers whereas S. mitis and S. oralis varied between 24-42% and 1-27% (mean values), respectively. The relative proportion of S. oralis increased significantly within the observation period while the proportion of S. salivarius and arginine-positive S. mitis showed a declining tendency. Actinomyces species adsorbed to the tooth surfaces within the first 4 h but did not increase their relative proportions until after 8-12 h, possibly due to a long doubling time. In one individual, encapsulated bacteria resembling Stomatococcus mucilaginosus were observed among the early colonizers. The time-dependent shifts in the bacterial populations within 24 h corroborate parallel ultrastructural findings.
本研究基于对1742株新鲜分离菌株的微生物学鉴定，描述了人类牙齿上主要的早期微生物群落。这些分离菌株取自四名牙科学生，他们将牙釉质和牙根表面的试件置于口腔中4、8、12和24小时。在实验期间，停止口腔卫生护理。在相同条件下，牙根表面的定植程度比牙釉质表面更严重。然而，微生物群落的组成是相同的。在最初的24小时内，微生物群落以链球菌和革兰氏阳性多形性杆菌为主。血链球菌仅占早期定植菌的6 - 18%，而缓症链球菌和口腔链球菌分别在24 - 42%和1 - 27%之间（平均值）变化。在观察期内，口腔链球菌的相对比例显著增加，而唾液链球菌和精氨酸阳性缓症链球菌的比例呈下降趋势。放线菌在最初4小时内吸附到牙齿表面，但直到8 - 12小时后其相对比例才增加，这可能是由于其较长的倍增时间。在一名个体中，在早期定植菌中观察到类似粘性口腔球菌的包膜细菌。24小时内细菌种群随时间的变化与平行的超微结构研究结果相符。
