人唾液中的α-D-甘露糖苷酶活性以及腮腺和颌下腺的酶产生速率。

Alpha-D-mannosidase activity in human saliva and rate of production of enzymes by parotid and submandibular glands.

作者信息

Engström P E, Söder P O, Olsson M L

机构信息

Department of Periodontology, Karolinska Institutet, Stockholm, Sweden.

出版信息

Scand J Dent Res. 1987 Oct;95(5):411-6. doi: 10.1111/j.1600-0722.1987.tb01632.x.

DOI:10.1111/j.1600-0722.1987.tb01632.x

PMID:3477855

Abstract

The aim of the investigation was to study alpha-D-mannosidase activity in samples of whole, parotid and submandibular saliva and the rate of production of alpha-D-mannosidases by parotid and submandibular glands. alpha-D-mannosidase activity was determined spectrophotometrically and spectrofluorimetrically. alpha-D-mannosidase activity was found at three different pH optima in whole saliva, around pH 4.8, 6.1 and 6.9. In parotid and submandibular saliva there were two different pH optima, around pH 4.8 and 6.1. The rate of production of alpha-D-mannosidase by parotid glands varied between 0.48 and 13.66 U/min and by submandibular glands between 0.25 and 2.52 U/min. The present study shows that two of the three alpha-D-mannosidases found in whole human saliva are produced by parotid and submandibular glands.

摘要

该研究的目的是研究全唾液、腮腺唾液和颌下唾液样本中的α-D-甘露糖苷酶活性，以及腮腺和颌下腺产生α-D-甘露糖苷酶的速率。采用分光光度法和荧光分光光度法测定α-D-甘露糖苷酶活性。在全唾液中发现α-D-甘露糖苷酶在三个不同的最适pH值下具有活性，分别在pH 4.8、6.1和6.9左右。在腮腺唾液和颌下唾液中存在两个不同的最适pH值，分别在pH 4.8和6.1左右。腮腺产生α-D-甘露糖苷酶的速率在0.48至13.66 U/分钟之间，颌下腺产生α-D-甘露糖苷酶的速率在0.25至2.52 U/分钟之间。本研究表明，在人类全唾液中发现的三种α-D-甘露糖苷酶中有两种是由腮腺和颌下腺产生的。