College of Plant Protection, Shandong Agricultural University, No. 61, Daizong Road, Taian, Shandong 271018, China.
Functional and Evolutionary Entomology, Terra, Gembloux Agro-Bio Tech, Liege University, Passage des Deportes 2, 5030 Gembloux, Belgium.
J Agric Food Chem. 2021 Nov 24;69(46):13734-13743. doi: 10.1021/acs.jafc.1c05978. Epub 2021 Nov 15.
(Fabricius) is a major insect pest of wheat worldwide that reduces crop yield and quality annually. Few germplasm resources with resistant genes to aphids have been identified and characterized. Here, octoploid Trititrigia, a species used in wheat distant hybridization breeding, was found to be repellent to after 2 year field investigations and associated with physiological and behavioral assays. Linalool monoterpene was identified to accumulate dominantly in plants in response to infestation. We cloned the resistance gene by assembling the transcriptome of aphid-infested or healthy octoploid Trititrigia. Functional characterization analysis indicated that encoded a terpene synthase and conferred resistance to by linalool emission before and after aphid feeding. Our study suggests that the octoploid Trititrigia with the aphid-resistant gene may have potential as a target resource for further breeding aphid-resistant wheat cultivars.
(Fabricius) 是一种世界性的小麦主要害虫，每年都会降低作物的产量和质量。目前已经鉴定和描述了少数具有抗蚜虫基因的种质资源。在这里，八倍体拟鹅观草是一种用于小麦远缘杂交育种的物种，经过 2 年的田间调查和相关的生理和行为测定，发现它对有驱避作用。芳樟醇单萜在受到 侵袭时会在植物中大量积累。我们通过组装受蚜虫侵袭或健康八倍体拟鹅观草的转录组克隆了抗性基因 。功能特征分析表明， 编码了一种萜烯合酶，通过在蚜虫取食前后释放芳樟醇而赋予对 的抗性。我们的研究表明，具有抗蚜基因 的八倍体拟鹅观草可能有潜力成为进一步培育抗蚜虫小麦品种的目标资源。
