Jiang Ya-Xiu, Chang Wen-Jing, Zhan Yi-di, Liu Zhen, Liu Yong
College of Plant Protection, Shandong Agricultural Univer-sity, Tai'an 271018, Shandong, China.
Ying Yong Sheng Tai Xue Bao. 2020 Oct;31(10):3248-3254. doi: 10.13287/j.1001-9332.202010.033.
We analyzed the resistance of wheat germplasm resources with different genetic backgrounds to aphids by fuzzy recognition technique in field and electrical penetration graph (EPG) in laboratory, with the aim to find new aphid-resistance resource. Results from a two-year field experiment showed that most trititrigia had medium and low resistance, while most triticale were medium and low susceptible. The trititrigia 21 (medium resistance), trititrigia 22 (low resistance), triticale 31 (medium susceptable) and triticale 32 (low susceptable) with stable resistance traits and diffe-rent resistance levels were selected for feeding behavior analysis of . We analyzed the basic waveforms, including non-feeding wave (Np), probing wave (P), potential drop (Pd), secretion of water saliva wave (E1), phloem feeding wave (E2), cellular mechanical hindrance wave (F), and xylem feeding wave (G). The time for initial probe of on trititrigia was significantly longer than that of triticale, while the duration of E1 wave on trititrigia was significantly longer than that of triticale. The duration of F wave on trititrigia 21 and the Np wave on the trititrigia 22 were the longest, while that of P wave on triticale 31 and the G wave on triticale 32 were the longest. Taking the durations of E1, F and Np waves as indices, the field identification on the resis-tance of wheat germplasm resources to aphid based on fuzzy recognition technique was basically consistent with that using EPG. Our results suggest that E1, F and Np waves could be used to evaluate the resistance levels of wheat to aphid. Tititrigia 21 and 22 were more resistant to aphid, which could be used as the germplasm for breeding aphid-resistance wheat varieties.
我们通过田间模糊识别技术和实验室电穿透图（EPG）分析了不同遗传背景的小麦种质资源对蚜虫的抗性，旨在寻找新的抗蚜资源。两年田间试验结果表明，多数八倍体小偃麦具有中低抗性，多数小黑麦具有中低感虫性。选择抗性性状稳定、抗性水平不同的八倍体小偃麦21（中抗）、八倍体小偃麦22（低抗）、小黑麦31（中感）和小黑麦32（低感）进行取食行为分析。我们分析了基本波形，包括非取食波（Np）、刺探波（P）、电位下降（Pd）、水唾液分泌波（E1）、韧皮部取食波（E2）、细胞机械阻碍波（F）和木质部取食波（G）。八倍体小偃麦初始刺探时间显著长于小黑麦，而八倍体小偃麦E1波持续时间显著长于小黑麦。八倍体小偃麦21的F波持续时间和八倍体小偃麦22的Np波持续时间最长，小黑麦31的P波持续时间和小黑麦32的G波持续时间最长。以E1、F和Np波持续时间为指标，基于模糊识别技术的小麦种质资源对蚜虫抗性的田间鉴定结果与EPG基本一致。我们的结果表明，E1、F和Np波可用于评价小麦对蚜虫的抗性水平。八倍体小偃麦21和22对蚜虫抗性较强，可作为抗蚜小麦品种选育的种质资源。
