Arand M, Robertson L W, Oesch F
Institute of Toxicology, University of Mainz, Federal Republic of Germany.
Anal Biochem. 1987 Jun;163(2):546-51. doi: 10.1016/0003-2697(87)90261-2.
A new, rapid, and sensitive method for assaying phenol sulfotransferase activity toward 2-naphthol is described. The product 2-naphthyl sulfate is quantitated fluorometrically. Optimal wavelengths for excitation and emission were determined by recording the three-dimensional fluorescence spectra of the substrate and the product. The new method is applicable to crude cell or tissue homogenates as well as to further purified preparations. A comparison to another widely used method is given to point out the advantages provided by the new procedure. In particular, sensitivity and accuracy of both methods are evaluated and the influence of interfering substances on both systems is compared. These results clearly indicate the superiority of the new method.
描述了一种用于测定苯酚磺基转移酶对2-萘酚活性的新的、快速且灵敏的方法。产物硫酸2-萘酯通过荧光法进行定量。通过记录底物和产物的三维荧光光谱确定了激发和发射的最佳波长。该新方法适用于粗细胞或组织匀浆以及进一步纯化的制剂。与另一种广泛使用的方法进行了比较，以指出新方法的优势。特别评估了两种方法的灵敏度和准确性，并比较了干扰物质对两个系统的影响。这些结果清楚地表明了新方法的优越性。
