de Lima Daniel Chaves, Medeiros Inácio Gomes, de Cássia Silva-Portela Rita, da Silva Junior Francisco Carlos, Fassarela Agnez-Lima Lucymara, de Souza Jorge Estefano Santana, Batistuzzo de Medeiros Silvia R
Instituto Federal de Educação, Ciência e Tecnologia do Rio Grande do Norte, CEP 59015-000, Natal, Brazil.
Laboratório de Biologia Molecular e Genômica, Universidade Federal do Rio Grande do Norte, CEP 59078-970, Natal, Brazil.
Can J Microbiol. 2022 Jan;68(1):45-54. doi: 10.1139/cjm-2021-0197. Epub 2021 Nov 15.
is an opportunistic pathogen found in tropical and subtropical regions worldwide. infections are difficult to treat, and many strains are resistant to antibiotics. Recently, a novel plasmid (pChV1) was discovered in the type strain ATCC 12472, suggesting that other strains may harbor extra-chromosomal DNA. The aim of the present study was to detect and compare new plasmids in Brazilian strains of using next-generation sequencing techniques. We obtained draft genomes of six plasmids from strains isolated from the Amazon region and aligned them with pChV1. At least three plasmids, CVAC05, CVACO2, and CVT8, were similar to pChV1. Phylogenetic analysis suggested that these new extra-chromosomal DNA sequences have a common origin with pChV1 but have diverged. Many of the ORFs detected were related to plasmid segregation/maintenance, viral structural proteins, and proteins with unknown functions. These findings may enable better genetic manipulation of , which will enhance our ability to exploit this valuable microorganism in industrial and clinical applications.
是一种在全球热带和亚热带地区发现的机会性致病菌。感染难以治疗，许多菌株对抗生素耐药。最近，在模式菌株ATCC 12472中发现了一种新型质粒（pChV1），这表明其他菌株可能含有染色体外DNA。本研究的目的是使用下一代测序技术检测和比较巴西菌株中的新质粒。我们从亚马逊地区分离的菌株中获得了六个质粒的草图基因组，并将它们与pChV1进行比对。至少三个质粒CVAC05、CVACO2和CVT8与pChV1相似。系统发育分析表明，这些新的染色体外DNA序列与pChV1有共同起源但已分化。检测到的许多开放阅读框与质粒分离/维持、病毒结构蛋白和功能未知的蛋白质有关。这些发现可能有助于对[具体微生物名称未给出]进行更好的基因操作，这将增强我们在工业和临床应用中利用这种有价值微生物的能力。
