State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, Shanghai, 200237, China.
Key Laboratory of Seawater Aquaculture, Zhejiang Marine Aquatic Research Institute, Zhoushan, 316100, China.
Fish Shellfish Immunol. 2022 Jan;120:133-138. doi: 10.1016/j.fsi.2021.11.008. Epub 2021 Nov 12.
Pseudomonas plecoglossicida is the causative agent of visceral granulomas disease (VGD) in large yellow croaker (LYC, Larimichthys crocea) farming. However, multi-antibiotic resistant of P. plecoglossicida creates an urgent need of an efficient vaccine to combat this pathogen. In this study, an inactivated vaccine added polyactin (PA), CpG-riched plasmid (pCpG) and aluminum adjuvant (Al) was developed. As a result, its relative percentage survival (RPS) against P. plecoglossicida were up to 64%. Comparatively, RPS of groups that vaccinated with vaccines adjuvanted with PA and Al or CpG and Al were 49% and 39%. However, an interesting result that the vaccine combined with PA, CpG and Al did not show the strongest activation of total serum protein and antibody levels in serum among three vaccinated groups. According to expressions of some cellular immune related genes, we found that the inactivated vaccine combined with PA, CpG and Al was more likely to induce a cellular immune response rather than humoral immune response. Totally, our study demonstrated that the mixture of PA, CpG and aluminum adjuvant is a potential adjuvant system for LYC vaccine development.
鮰爱德华氏菌是大黄鱼（Larimichthys crocea）内脏肉芽肿病（VGD）的病原体。然而，鮰爱德华氏菌对抗生素的多重耐药性使得开发一种有效的疫苗来对抗这种病原体变得非常迫切。在本研究中，开发了一种添加聚肌苷酸（PA）、CpG 丰富质粒（pCpG）和铝佐剂（Al）的灭活疫苗。结果表明，该疫苗对鮰爱德华氏菌的相对存活率（RPS）高达 64%。相比之下，用添加 PA 和 Al 或 CpG 和 Al 的疫苗免疫的组的 RPS 分别为 49%和 39%。然而，一个有趣的结果是，在三种免疫组中，添加 PA、CpG 和 Al 的疫苗并没有显示出最强的总血清蛋白和抗体水平的激活。根据一些细胞免疫相关基因的表达，我们发现添加 PA、CpG 和 Al 的灭活疫苗更有可能诱导细胞免疫反应而不是体液免疫反应。总的来说，我们的研究表明，PA、CpG 和铝佐剂的混合物是大黄鱼疫苗开发的一种有潜力的佐剂系统。
