State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, Shanghai 200237, China.
Southern Marine Science and Engineering Guangdong Laboratory (Zhuhai), Zhuhai 519000, China.
Fish Shellfish Immunol. 2024 Jan;144:109243. doi: 10.1016/j.fsi.2023.109243. Epub 2023 Nov 22.
Large yellow croaker (Larimichthys crocea) farm industry in China suffered from huge economic loss caused by Pseudomonas plecoglossicida infection. Due to multi-antibiotic resistance, efficient vaccines are urgent to be developed to combat this pathogen. In this study, an inactivated vaccine was developed with an aluminium adjuvant (Alum) plus ginseng stem and leaf saponins (GSLS). As a result, the relative percentage survival (RPS) against P. plecoglossicida was up to 67.8 %. Comparatively, RPS of groups that vaccinated with only inactivated vaccine and vaccine containing Alum or Montanide™ 763A as adjuvant were 21.8 %, 32.2 % and 62.1 %, respectively. Assays for total serum protein and serum lysozyme activity in group vaccinated with inactivated vaccine plus Alum + GSLS adjuvant were significantly higher than that in control group. Moreover, specific antibody in serum elicited a rapid and persistent level. According to the expression of some immune related genes, inactivated vaccine plus Alum + GSLS adjuvant induced a stronger cellular immune response which was vital to defend against P. plecoglossicida. In conclusion, our study demonstrated that the compound Alum and GSLS adjuvant is a potential adjuvant system to develop LYC vaccine.
中国大黄鱼（Larimichthys crocea）养殖业因感染假单胞菌（Pseudomonas plecoglossicida）而遭受巨大经济损失。由于存在多种抗生素耐药性，迫切需要开发有效的疫苗来对抗这种病原体。在本研究中，使用铝佐剂（Alum）和人参茎叶皂苷（GSLS）开发了一种灭活疫苗。结果，对 P. plecoglossicida 的相对存活率（RPS）高达 67.8%。相比之下，仅接种灭活疫苗和含有 Alum 或 Montanide™ 763A 作为佐剂的疫苗的组的 RPS 分别为 21.8%、32.2%和 62.1%。接种灭活疫苗加 Alum+GSLS 佐剂组的总血清蛋白和血清溶菌酶活性测定明显高于对照组。此外，血清中特异性抗体迅速产生并持续存在。根据一些免疫相关基因的表达，灭活疫苗加 Alum+GSLS 佐剂诱导了更强的细胞免疫反应，这对于抵抗 P. plecoglossicida 至关重要。总之，本研究表明，复合 Alum 和 GSLS 佐剂是开发 LYC 疫苗的一种有潜力的佐剂系统。
