Department of Laboratory Medicine, Qilu Hospital (Qingdao), Cheeloo College of Medicine, Shandong University, Qingdao, Shandong, China.
Department of Internal Medicine, Qilu Hospital of Shandong University, Jinan, Shandong, China.
Immun Inflamm Dis. 2022 Feb;10(2):235-240. doi: 10.1002/iid3.567. Epub 2021 Nov 16.
Mast cells are regarded as a kind of classical anaphylaxis cells. However autoimmune diseases and allergic reactions have many similarities or overlaps. A large number of papers have proved that mast cells play a significant role in the pathogenesis of systemic lupus erythematosus (SLE). It is speculated that IgE, anti-IgE antibodies, FcεRI, and anti-FcεRI antibodies activate mast cells through autoimmune pathways and participate in the disease process of SLE. Naturally occurring protein molecules not only exist in monomer form, but also in polymer of protein molecules. Therefore, whether IgE, FcεRIα, anti-IgE antibodies, and anti-FcεRI antibodies also exist in polymeric forms in the natural state is worthy of further investigation.
The serum samples and clinical data of 131 patients with SLE were collected from Qilu Hospital (Qingdao). Sixty healthy individuals were collected as the control group. Serum FcεRIα, anti-IgE, and anti-FcεRI were detected by enzyme-linked immunosorbent assay. Serum IgE was detected by rate scatter nephelometry. A Chinese hamster ovarian cancer cell line CHO3D10 transfected with human FcεRIα was cultured and the cell protein extract was prepared. The existence forms of FcεRIα in the cell protein extract were detected by the native-page method.
The serum FcεRIα in SLE patients was significantly higher than that in control group (3.52 [2.18, 4.71] µg/ml and 1.87 [1.52, 2.33] µg/ml, respectively; p < .05). Anti-IgE was significantly lower than that in the control group (0.85 [0.55, 1.21] µg/ml and 1.23 [0.95, 1.58] µg/ml, respectively; p < .05). The CHO3D10 cell line expressed the FcεRIα, which had one kind of monomer (mFcεRIα) and two kinds of polymers (pFcεRIα) in the degeneration conditions.
In patients with SLE, the expression of FcεRIα was increased and the level of anti-IgE was decreased. FcεRIα had one kind of monomer and two kinds of polymers. Mast cell-associated FcεRIα involved in the inflammatory lesion of SLE.
肥大细胞被认为是一种经典的过敏细胞。然而，自身免疫性疾病和过敏反应有许多相似之处或重叠。大量的论文已经证明，肥大细胞在系统性红斑狼疮（SLE）的发病机制中起着重要作用。据推测，IgE、抗 IgE 抗体、FcεRI 和抗 FcεRI 抗体通过自身免疫途径激活肥大细胞，并参与 SLE 的疾病过程。天然存在的蛋白质分子不仅以单体形式存在，而且还以蛋白质分子的聚合物形式存在。因此，IgE、FcεRIα、抗 IgE 抗体和抗 FcεRI 抗体在自然状态下是否也以聚合形式存在，值得进一步研究。
收集齐鲁医院（青岛）131 例 SLE 患者的血清样本和临床资料，收集 60 例健康个体作为对照组。采用酶联免疫吸附试验检测血清 FcεRIα、抗 IgE 和抗 FcεRI。采用速率散射比浊法检测血清 IgE。培养转染人 FcεRIα 的中国仓鼠卵巢癌细胞系 CHO3D10，并制备细胞蛋白提取物。采用天然电泳法检测细胞蛋白提取物中 FcεRIα 的存在形式。
SLE 患者血清 FcεRIα 明显高于对照组（分别为 3.52[2.18，4.71]μg/ml 和 1.87[1.52，2.33]μg/ml，p<0.05）。抗 IgE 明显低于对照组（分别为 0.85[0.55，1.21]μg/ml 和 1.23[0.95，1.58]μg/ml，p<0.05）。CHO3D10 细胞系在变性条件下表达 FcεRIα，具有一种单体（mFcεRIα）和两种聚合物（pFcεRIα）。
SLE 患者 FcεRIα 表达增加，抗 IgE 水平降低。FcεRIα 有一种单体和两种聚合物。与肥大细胞相关的 FcεRIα 参与了 SLE 的炎症损伤。
