Emodin-Conjugated PEGylation of FeO Nanoparticles for FI/MRI Dual-Modal Imaging and Therapy in Pancreatic Cancer.

BACKGROUND

Pancreatic cancer (PC) remains a difficult tumor to diagnose and treat. It is often diagnosed as advanced by reason of the anatomical structure of the deep retroperitoneal layer of the pancreas, lack of typical symptoms and effective screening methods to detect this malignancy, resulting in a low survival rate. Emodin (EMO) is an economical natural product with effective treatment and few side effects of cancer treatment. Magnetic nanoparticles (MNPs) can achieve multiplexed imaging and targeted therapy by loading a wide range of functional materials such as fluorescent dyes and therapeutic agents.

PURPOSE

The purpose of this study was to design and evaluate a multifunctional theranostic nanoplatform for PC diagnosis and treatment.

METHODS

In this study, we successfully developed EMO-loaded, Cy7-functionalized, PEG-coated FeO (FeO-PEG-Cy7-EMO). Characteristics including morphology, hydrodynamic size, zeta potentials, stability, and magnetic properties of FeO-PEG-Cy7-EMO were evaluated. Fluorescence imaging (FI)/magnetic resonance imaging (MRI) and therapeutic treatment were examined in vitro and in vivo.

RESULTS

FeO-PEG-Cy7-EMO nanoparticles had a core size of 9.9 ± 1.2 nm, which showed long-time stability and FI/MRI properties. Bio-transmission electron microscopy (bio-TEM) results showed that FeO-PEG-Cy7-EMO nanoparticles were endocytosed into BxPC-3 cells, while few were observed in hTERT-HPNE cells. Prussian blue staining also confirmed that BxPC-3 cells have a stronger phagocytic ability as compared to hTERT-HPNE cells. Additionally, FeO-PEG-Cy7-EMO had a stronger inhibition effect on BxPC-3 cells than FeO-PEG and EMO. The hemolysis experiment proved that FeO-PEG-Cy7-EMO can be used in vivo experiments. In vivo analysis demonstrated that FeO-PEG-Cy7-EMO enabled FI/MRI dual-modal imaging and targeted therapy in pancreatic tumor xenografted mice.

CONCLUSION

FeO-PEG-Cy7-EMO may serve as a potential theranostic nanoplatform for PC.