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RHPN1-AS1 通过海绵吸附 microRNA-485-5p 并释放 DNA 拓扑异构酶 IIα（）促进卵巢癌发生。

Rhophilin rho GTPase binding protein 1-antisense RNA 1 (RHPN1-AS1) promotes ovarian carcinogenesis by sponging microRNA-485-5p and releasing DNA topoisomerase II alpha ().

机构信息

Hunan Key Laboratory of the Research and Development of Novel Pharmaceutical Preparations, Changsha, Hunan, China.

Academician Workstation, Changsha Medical University, Changsha, Hunan, China.

出版信息

Bioengineered. 2021 Dec;12(2):12003-12022. doi: 10.1080/21655979.2021.2002494.

DOI:10.1080/21655979.2021.2002494
PMID:34787052
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC8810118/
Abstract

Ovarian cancer (OC) is the most common and lethal gynecological cancer worldwide. Long non-coding RNAs (lncRNAs) and sponging microRNAs (miRNAs) serve as key regulators in the biological processes of OC. We sought to evaluate the effect of the RHPN1-AS1-miR-485-5p-DNA topoisomerase II alpha (TOP2A) axis in regulating OC progression. RHPN1-AS1, miR-485-5p, and TOP2A levels in OC tissues and cells were determined by RT-qPCR. The interaction of RHPN1-AS1/miR-485-5p/TOP2A was assessed using luciferase, RNA immunoprecipitation, and RNA pull-down assays. RHPN1-AS1 silencing allowed us to explore its biological function by measuring cell viability, proliferation, migration, invasion, and apoptosis in OC cells. experiments were performed to verify the findings. We found that the RHPN1-AS1 and TOP2A levels were significantly enhanced, whereas the miR-485-5p levels were reduced in OC tissues and cells. RHPN1-AS1 silencing attenuated cell growth, facilitated apoptosis in OC cells, and inhibited tumor growth . Notably, RHPN1-AS1 negatively regulating miR-485-5p promoted the TOP2A expression in OC cells. In conclusion, RHPN1-AS1 sponging miR-485-5p accelerated the progression of OC by elevating TOP2A expression, which makes it a promising target for the treatment of OC patients.

摘要

卵巢癌（OC）是全球最常见和致命的妇科癌症。长链非编码 RNA（lncRNA）和海绵 microRNA（miRNA）作为 OC 生物学过程的关键调节剂。我们试图评估 RHPN1-AS1-miR-485-5p-DNA 拓扑异构酶 II 阿尔法（TOP2A）轴在调节 OC 进展中的作用。通过 RT-qPCR 测定 OC 组织和细胞中的 RHPN1-AS1、miR-485-5p 和 TOP2A 水平。使用荧光素酶、RNA 免疫沉淀和 RNA 下拉测定评估 RHPN1-AS1/miR-485-5p/TOP2A 的相互作用。通过测量 OC 细胞中的细胞活力、增殖、迁移、侵袭和凋亡，沉默 RHPN1-AS1 来探索其生物学功能。进行了 实验来验证这些发现。我们发现，在 OC 组织和细胞中，RHPN1-AS1 和 TOP2A 水平显著升高，而 miR-485-5p 水平降低。RHPN1-AS1 沉默减弱了 OC 细胞的生长，促进了 OC 细胞的凋亡，并抑制了肿瘤的生长。值得注意的是，RHPN1-AS1 负调控 miR-485-5p 促进了 OC 细胞中 TOP2A 的表达。总之，RHPN1-AS1 海绵 miR-485-5p 通过提高 TOP2A 的表达加速了 OC 的进展，这使其成为治疗 OC 患者的有前途的靶点。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3206/8810118/c2fa3c827d46/KBIE_A_2002494_F0009_OC.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3206/8810118/b578cc2f7d24/KBIE_A_2002494_F0001_OC.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3206/8810118/8cd74222290f/KBIE_A_2002494_F0002_OC.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3206/8810118/5b0b11a20e1a/KBIE_A_2002494_F0003_OC.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3206/8810118/0f06204680e3/KBIE_A_2002494_F0004_OC.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3206/8810118/b4d02ec1fff2/KBIE_A_2002494_F0005_OC.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3206/8810118/33a36d3ad767/KBIE_A_2002494_F0006_OC.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3206/8810118/858969e60bd3/KBIE_A_2002494_F0007_OC.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3206/8810118/68aeae5bdc09/KBIE_A_2002494_F0008_OC.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3206/8810118/c2fa3c827d46/KBIE_A_2002494_F0009_OC.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3206/8810118/b578cc2f7d24/KBIE_A_2002494_F0001_OC.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3206/8810118/8cd74222290f/KBIE_A_2002494_F0002_OC.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3206/8810118/5b0b11a20e1a/KBIE_A_2002494_F0003_OC.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3206/8810118/0f06204680e3/KBIE_A_2002494_F0004_OC.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3206/8810118/b4d02ec1fff2/KBIE_A_2002494_F0005_OC.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3206/8810118/33a36d3ad767/KBIE_A_2002494_F0006_OC.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3206/8810118/858969e60bd3/KBIE_A_2002494_F0007_OC.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3206/8810118/68aeae5bdc09/KBIE_A_2002494_F0008_OC.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3206/8810118/c2fa3c827d46/KBIE_A_2002494_F0009_OC.jpg

相似文献

[1]
Rhophilin rho GTPase binding protein 1-antisense RNA 1 (RHPN1-AS1) promotes ovarian carcinogenesis by sponging microRNA-485-5p and releasing DNA topoisomerase II alpha ().

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[2]
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[3]
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[5]
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[6]
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[7]
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[8]
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[10]
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引用本文的文献

[1]
Overexpression of FOS enhances the malignant potential of eutopic endometrial stromal cells in patients with endometriosis‑associated ovarian cancer.

Oncol Rep. 2025-4

[2]
TOP2A deficiency leads to human recurrent spontaneous abortion and growth retardation of mouse pre-implantation embryos.

Mol Med. 2022-12-30

本文引用的文献

[1]
lncRNA RHPN1-AS1 promotes the progression of endometrial cancer through the activation of ERK/MAPK pathway.

J Obstet Gynaecol Res. 2021-2

[2]
Profiling of specific long non-coding RNA signatures identifies ST8SIA6-AS1 AS a novel target for breast cancer.

J Gene Med. 2021-2

[3]
Circ-HIPK2 Accelerates Cell Apoptosis and Autophagy in Myocardial Oxidative Injury by Sponging miR-485-5p and Targeting ATG101.

J Cardiovasc Pharmacol. 2020-10

[4]
High expression of TOP2A in hepatocellular carcinoma is associated with disease progression and poor prognosis.

Oncol Lett. 2020-11

[5]
LncRNA FAM230B Promotes Gastric Cancer Growth and Metastasis by Regulating the miR-27a-5p/TOP2A Axis.

Dig Dis Sci. 2021-8

[6]
Long noncoding RNAs in cancer immunity: a new avenue in drug discovery.

Drug Discov Today. 2021-1

[7]
LINC01224 Exhibits Cancer-Promoting Activity in Epithelial Ovarian Cancer Through microRNA-485-5p-Mediated PAK4 Upregulation.

Onco Targets Ther. 2020-6-16

[8]
lncRNA RHPN1-AS1 Promotes Ovarian Cancer Growth and Invasiveness Through Inhibiting miR-1299.

Onco Targets Ther. 2020-6-10

[9]
Non-coding RNAs are involved in the response to oxidative stress.

Biomed Pharmacother. 2020-7

[10]
TOP2A Promotes Cell Migration, Invasion and Epithelial-Mesenchymal Transition in Cervical Cancer via Activating the PI3K/AKT Signaling.

Cancer Manag Res. 2020-5-21

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