Zhao Lin, Liu Ting, Zhang Xingna, Zuo Donghua, Liu Chunna
Department of Gynaecology, Linyi Cancer Hospital, Linyi 276000, People's Republic of China.
Onco Targets Ther. 2020 Jun 10;13:5337-5344. doi: 10.2147/OTT.S248050. eCollection 2020.
Ovarian cancer (OC) is a big threat for public health. However, the molecular mechanism underlying OC development and progression remains unclear. Although the importance of lncRNA in cancer has been proven, how lncRNA is involved in OC is waiting for further investigation.
qRT-PCR was performed to test expression level. CCK8 and colony formation were conducted to analyze proliferation. Transwell was conducted to measure migration and invasion. Luciferase reporter assay and pulldown assay were utilized to validate RNA interaction.
lncRNA RHPN1-AS1 was highly expressed in OC tissues. RHPN1-AS1 was positively correlated with OC progression and its high expression indicated a low survival rate. Moreover, knockdown of RHPN1-AS1 significantly inhibited the proliferation, migration and invasion of OC cells, and bioinformatics analysis identified that miR-1299 was sponged by RHPN1-AS1 in OC cells. Knockdown of RHPN1-AS1 markedly promoted miR-1299 expression. Of note, inhibition of miR-1299 reversed the roles of RHPN1-AS1 silencing on suppressing proliferation, migration and invasion.
Our study demonstrates that RHPN1-AS1 promotes OC progression via sponging miR-1299, suggesting RHPN1-AS1 may be a novel therapeutic target.
卵巢癌（OC）对公众健康构成重大威胁。然而，OC发生和进展的分子机制仍不清楚。尽管长链非编码RNA（lncRNA）在癌症中的重要性已得到证实，但lncRNA如何参与OC仍有待进一步研究。
采用qRT-PCR检测表达水平。进行CCK8和集落形成实验分析增殖情况。采用Transwell实验检测迁移和侵袭能力。利用荧光素酶报告基因实验和下拉实验验证RNA相互作用。
lncRNA RHPN1-AS1在OC组织中高表达。RHPN1-AS1与OC进展呈正相关，其高表达提示生存率低。此外，敲低RHPN1-AS1显著抑制OC细胞的增殖、迁移和侵袭，生物信息学分析确定在OC细胞中miR-1299被RHPN1-AS1吸附。敲低RHPN1-AS1明显促进miR-1299表达。值得注意的是，抑制miR-1299可逆转RHPN1-AS1沉默对增殖、迁移和侵袭的抑制作用。
我们的研究表明，RHPN1-AS1通过吸附miR-1299促进OC进展，提示RHPN1-AS1可能是一个新的治疗靶点。
