Postgraduate Program in Oral Pathology, Federal University of Rio Grande do Norte, Natal, RN, Brazil.
Dentistry Department, State University of Paraíba, Campina Grande, PB, Brazil.
Braz Dent J. 2021 Jul-Aug;32(4):74-82. doi: 10.1590/0103-6440202104279.
The Inhibitor of Growth (ING) gene family is a group of tumor suppressor genes that play important roles in cell cycle control, senescence, DNA repair, cell proliferation, and apoptosis. However, inactivation and downregulation of these proteins have been related in some neoplasms. The present study aimed to evaluate the immunohistochemical profiles of ING3 and ING4 proteins in a series of benign epithelial odontogenic lesions.
The sample comprised of 20 odontogenic keratocysts (OKC), 20 ameloblastomas (AM), and 15 adenomatoid odontogenic tumors (AOT) specimens. Nuclear and cytoplasmic immunolabeling of ING3 and ING4 were semi-quantitatively evaluated in epithelial cells of the odontogenic lesions, according to the percentage of immunolabelled cells in each case. Descriptive and statistics analysis were computed, and the p-value was set at 0.05.
No statistically significant differences were found in cytoplasmic and nuclear ING3 immunolabeling among the studied lesions. In contrast, AOTs presented higher cytoplasmic and nuclear ING4 labeling compared to AMs (cytoplasmic p-value = 0.01; nuclear p-value < 0.001) and OKCs (nuclear p-value = 0.007).
ING3 and ING4 protein downregulation may play an important role in the initiation and progression of more aggressive odontogenic lesions, such as AMs and OKCs.
生长抑制剂（ING）基因家族是一组肿瘤抑制基因，它们在细胞周期控制、衰老、DNA 修复、细胞增殖和细胞凋亡中发挥重要作用。然而，这些蛋白质的失活和下调与一些肿瘤有关。本研究旨在评估 ING3 和 ING4 蛋白在一系列良性上皮性牙源性病变中的免疫组织化学特征。
该样本包括 20 例牙源性角化囊肿（OKC）、20 例造釉细胞瘤（AM）和 15 例腺牙源性肿瘤（AOT）标本。根据每个病例免疫标记细胞的百分比，对牙源性病变上皮细胞中的 ING3 和 ING4 的核和细胞质免疫标记进行半定量评估。进行描述性和统计学分析，p 值设为 0.05。
在所研究的病变中，细胞质和核 ING3 免疫标记没有统计学差异。相比之下，AOT 与 AM（细胞质 p 值=0.01；核 p 值<0.001）和 OKC（核 p 值=0.007）相比，具有更高的细胞质和核 ING4 标记。
ING3 和 ING4 蛋白下调可能在更具侵袭性的牙源性病变（如 AM 和 OKC）的发生和进展中发挥重要作用。
