Tenório J-R, Santana T, Queiroz S-I-M-L, de Oliveira D-H-I-P, Queiroz L-M-G
Departamento de Odontologia, Universidade Federal do Rio Grande do Norte, Av. Senador Salgado Filho, 1787, Lagoa Nova, CEP 59056-000 Natal, RN, Brasil,
Med Oral Patol Oral Cir Bucal. 2018 Mar 1;23(2):e120-e125. doi: 10.4317/medoral.22019.
Ameloblastoma (AMB), odontogenic keratocyst (OKC) and adenomatoid odontogenic tumor (AOT) are epithelial odontogenic lesions with diverse biologic profiles. Defects in regulation of apoptosis and cell cycle may be involved in the development and progression of those lesions, therefore we aimed to investigate the expression of Bcl-2, Bax and p53 to better understand the possible role of these proteins in AMBs, OKCs and AOTs.
The studied sample consisted of 20 AMBs, 20 OKCs and 20 AOTs. Immunohistochemistry technique was performed for the antibodies p53, Bcl-2 and Bax. Immunoreactivity was observed in the epithelial component and positive cells were counted in five fields (100x magnification). Statistical analysis was performed with Kruskal-Wallis and Spearman tests (p <0.05).
All lesions exhibited staining for the three studied proteins. There was no statistically significant associations between the expression of proteins and the lesions, however we identified a positive correlation between the expression of p53 and Bcl-2 (r = 0.200) and a negative correlation between p53 and Bax expressions (r = -0.100). In addition, p53 and Bax were similarly expressed between AMBs and OKCs. Bcl-2 was similarly expressed in AMBs and AOTs.
Apoptosis regulatory proteins, as well as cell cycle proteins, are differently expressed in epithelial odontogenic lesions and their expression is possibly related to the biological behavior of AMB, OKC and AOT.
成釉细胞瘤（AMB）、牙源性角化囊肿（OKC）和腺样牙源性肿瘤（AOT）是具有不同生物学特征的上皮性牙源性病变。细胞凋亡和细胞周期调控缺陷可能参与这些病变的发生和发展，因此我们旨在研究Bcl-2、Bax和p53的表达，以更好地了解这些蛋白质在AMB、OKC和AOT中的可能作用。
研究样本包括20例AMB、20例OKC和20例AOT。对p53、Bcl-2和Bax抗体进行免疫组织化学技术检测。在上皮成分中观察免疫反应性，并在五个视野（100倍放大）中对阳性细胞进行计数。采用Kruskal-Wallis和Spearman检验进行统计学分析（p<0.05）。
所有病变均显示出三种研究蛋白质的染色。蛋白质表达与病变之间无统计学显著相关性，然而我们发现p53和Bcl-2表达之间呈正相关（r = 0.200），p53和Bax表达之间呈负相关（r = -0.100）。此外，AMB和OKC之间p53和Bax的表达相似。Bcl-2在AMB和AOT中的表达相似。
凋亡调节蛋白以及细胞周期蛋白在上皮性牙源性病变中表达不同，其表达可能与AMB、OKC和AOT的生物学行为有关。
