School of Food and Wine Science, Ningxia University, Yinchuan, 750021, People's Republic of China.
J Food Prot. 2022 Mar 1;85(3):390-397. doi: 10.4315/JFP-21-354.
This study was conducted to determine the sterilization effect of a combination of high pressure thermal sterilization (HPTS) and ε-polylysine (ε-PL) on Bacillus subtilis spores. The spores were treated with HPTS (550 MPa at 25, 65, and 75°C) and ε-PL at 0.1 and 0.3%. HPTS and ε-PL synergistically decreased the number of surviving spores and increased the release of the intracellular components in the spore suspension, with the maximal effects from treatment with 550 MPa at 75°C plus 0.3% ε-PL. Maximum fluidity and permeability of the cell inner membrane were observed with 550 MPa at 75°C plus 0.3% ε-PL. Changes in membrane lipids were detected from 3,000 to 2,800 cm-1 by Fourier transform infrared spectroscopy. The results provide new insights into the mechanism by which HPTS and ε-PL synergistically sterilize B. subtilis spores.
本研究旨在确定高压热灭菌（HPTS）和ε-聚赖氨酸（ε-PL）联合作用对枯草芽孢杆菌孢子的灭菌效果。将孢子用 HPTS（25、65 和 75°C 时 550 MPa）和 0.1%和 0.3%的 ε-PL 处理。HPTS 和 ε-PL 协同降低了存活孢子的数量，并增加了孢子悬浮液中细胞内成分的释放，在 75°C 时用 550 MPa 加 0.3%ε-PL 处理的效果最大。用 550 MPa 在 75°C 加 0.3%ε-PL 观察到细胞内膜的最大流动性和渗透性。傅里叶变换红外光谱检测到膜脂从 3000 到 2800 cm-1 的变化。这些结果为 HPTS 和 ε-PL 协同灭菌枯草芽孢杆菌孢子的机制提供了新的见解。
