Liu Long, Yu Huan, Wang Dun
State Key Laboratory of Crop Stress Biology for Arid Areas, Northwest A&F University, Yangling, Shaanxi 712100, PR China.
State Key Laboratory of Crop Stress Biology for Arid Areas, Northwest A&F University, Yangling, Shaanxi 712100, PR China.
Virus Res. 2022 Jan 15;308:198630. doi: 10.1016/j.virusres.2021.198630. Epub 2021 Nov 14.
The oak lappet moth, Trabala vishnou gigantina is a forest insect pest that damages broad-leaf trees severely. Trabala vishnou gigantina nucleopolyhedrovirus (TrviNPV) has been isolated from a naturally infected T. vishnou gigantina larva and investigated for its biology and the potential to be a biological control agent against its insect host. TrviNPV was characterized by electron microscope of occlusion bodies (OBs), genomic sequencing and field control efficacy. TrviNPV OBs exhibited an irregular polyhedral shape varying in size from 0.99 to 3.99 μm with multiple nucleocapsids per virion. The genome of this virus was 165 657 bp in length with 40.33% GC content and encoded 146 putative ORFs including the 38 baculovirus core genes. TrviNPV is a group II alphabaculovirus that encodes F protein and lacks the gp64 gene specific to group I alphabaculoviruses. Phylogeny and Kimura-2 parameter analysis revealed TrviNPV to be a novel species and closest to ArdiNPV, EupsNPV and OrleNPV. Bioassays and field trials in a shrubland revealed that TrviNPV was virulent and effective to control T. vishnou gigantina in arid semi-desert region. This work firstly reported the whole genome of TrviNPV as well as its biological characters for a possibility to develop this virus as bio-pesticide in the future.
栎黄枯叶蛾（Trabala vishnou gigantina）是一种严重危害阔叶树的森林害虫。栎黄枯叶蛾核型多角体病毒（TrviNPV）已从自然感染的栎黄枯叶蛾幼虫中分离出来，并对其生物学特性以及作为其昆虫宿主的生物防治剂的潜力进行了研究。通过多角体（OBs）的电子显微镜观察、基因组测序和田间防治效果对TrviNPV进行了表征。TrviNPV的多角体呈不规则多面体形状，大小在0.99至3.99μm之间变化，每个病毒粒子含有多个核衣壳。该病毒的基因组长度为165657bp，GC含量为40.33%，编码146个推定的开放阅读框，包括38个杆状病毒核心基因。TrviNPV是一种II组α杆状病毒，编码F蛋白，缺乏I组α杆状病毒特有的gp64基因。系统发育和Kimura-2参数分析表明，TrviNPV是一个新物种，与ArdiNPV、EupsNPV和OrleNPV最为接近。在灌木丛中的生物测定和田间试验表明，TrviNPV对干旱半荒漠地区的栎黄枯叶蛾具有毒性且防治效果良好。这项工作首次报道了TrviNPV的全基因组及其生物学特性，为未来将该病毒开发为生物农药提供了可能性。
