Mao Jing, Zhang Zhuchi, Chen Yongde, Wu Tao, Fersht Viktor, Jin Yan, Meng Jing, Zhang Min
State Key Laboratory of Food Nutrition and Safety, Food Biotechnology Engineering Research Center of Ministry of Education, College of food Science and Engineering, Tianjin University of Science & Technology, Tianjin 300457, China.
Bestlife Biological Technology Co., Ltd, Hebei Province, China.
Food Funct. 2021 Dec 13;12(24):12362-12371. doi: 10.1039/d1fo02267a.
Lung cancer is a common cancer with high mortality worldwide, and non-small cell lung cancer (NSCLC) accounts for the majority. The clinical treatment effect of NSCLC is not ideal. The aim of this study was to investigate the inhibitory effect of sea cucumber peptide (SCP) on NSCLC and its mechanism. The results showed that SCP could effectively inhibit the proliferation, migration and invasion of A549 cells. In addition, SCP can also inhibit the formation of pleural effusion and tumor growth in lung cancer mice, reduce liver and kidney injury, increase the levels of IL-2 and IL-12, decrease the levels of IL-6 and TNF-α, and prolong the survival time of mice. The microRNA sequencing and immunohistochemistry of mouse tumors showed that the tumor suppressor gene TUSC2 targeted by miR-378a-5p was involved in the inhibition of tumor growth by SCP. This study provides an experimental basis for the further development of SCP as an anti-tumor nutritional supplement, and provides a new idea for exploring the molecular mechanism of food derived active peptides in anti-tumor applications.
肺癌是全球范围内一种常见且死亡率高的癌症，其中非小细胞肺癌（NSCLC）占大多数。NSCLC的临床治疗效果并不理想。本研究旨在探讨海参肽（SCP）对NSCLC的抑制作用及其机制。结果表明，SCP能有效抑制A549细胞的增殖、迁移和侵袭。此外，SCP还可抑制肺癌小鼠胸腔积液的形成和肿瘤生长，减轻肝肾损伤，提高IL-2和IL-12水平，降低IL-6和TNF-α水平，并延长小鼠存活时间。对小鼠肿瘤的微小RNA测序和免疫组化显示，miR-378a-5p靶向的肿瘤抑制基因TUSC2参与了SCP对肿瘤生长的抑制作用。本研究为SCP作为抗肿瘤营养补充剂的进一步开发提供了实验依据，并为探索食物源活性肽在抗肿瘤应用中的分子机制提供了新思路。
