Guangdong Province Key Laboratory for Biotechnology Drug Candidates, School of Biosciences and Biopharmaceutics, Guangdong Pharmaceutical University, Guangzhou 510006, China.
College of Marine Sciences, South China Agricultural University, Guangzhou 510640, China.
Nutrients. 2022 Feb 13;14(4):786. doi: 10.3390/nu14040786.
Cancer remains the primary cause of death worldwide. To develop less toxic anti-cancer drugs to relieve the suffering and improve the survival of cancer patients is the major focus in the anti-cancer field. To this end, marine creatures are being extensively studied for their anti-cancer effects, since extracts from at least 10% of the marine organisms have been shown to possess anti-tumor activities. As a classic Chinese traditional medicine, sea cucumbers and compounds extracted from the sea cucumbers, such as polysaccharides and saponins, have recently been shown to exhibit anti-cancer, anti-inflammatory, and anti-oxidant effects. () is a tropical edible sea cucumber species that has been successfully cultivated and farmed in large scales, providing a readily available source of raw materials to support the development of novel marine anti-cancer drugs. However, very few studies have so far been performed on the biological activities of . In this study, we first investigated the anti-cancer effect of protein on three cancer cell lines (i.e., HepG2, A549, Panc02) and three normal cell lines (NIH-3T3, HaCaT, 16HBE). Our data showed that protein decreased the cell viabilities of HepG2, A549, HaCaT, 16HBE in a concentration-dependent manner, while Panc02 and NIH-3T3 in a time- and concentration-dependent manner. We also found that the inhibitory effect of protein (≥10 μg/mL) on cell viability is near or even superior to EPI, a clinical chemotherapeutic agent. In addition, our data also demonstrated that protein significantly affected the cell cycle and induced apoptosis in the three cancer cell lines investigated; in comparison, it showed no effects on the normal cell lines (i.e., NIH-3T3, HaCaT and 16HBE). Finally, our results also showed that protein exhibited the excellent performance in inhibiting cell immigrations. In conclusion, protein targeted the cancer cell cycles and induced cancer cell apoptosis; its superiority to inhibit cancer cell migration compared with EPI, shows the potential as a promising anti-cancer drug.
癌症仍然是全球主要的死亡原因。开发毒性更低的抗癌药物，以减轻癌症患者的痛苦并提高其生存率，是抗癌领域的主要关注点。为此，人们正在广泛研究海洋生物的抗癌作用，因为至少有 10%的海洋生物提取物具有抗肿瘤活性。海参作为一种经典的传统中药，以及从海参中提取的化合物，如多糖和皂苷，最近已被证明具有抗癌、抗炎和抗氧化作用。()是一种热带食用海参，已成功进行大规模养殖，为开发新型海洋抗癌药物提供了现成的原料来源。然而，迄今为止，对 的生物学活性研究甚少。在这项研究中，我们首先研究了 蛋白对三种癌细胞系（即 HepG2、A549、Panc02）和三种正常细胞系（NIH-3T3、HaCaT、16HBE）的抗癌作用。我们的数据表明， 蛋白以浓度依赖性方式降低 HepG2、A549、HaCaT、16HBE 的细胞活力，而以时间和浓度依赖性方式降低 Panc02 和 NIH-3T3 的细胞活力。我们还发现， 蛋白（≥10μg/ml）对细胞活力的抑制作用接近甚至优于临床化疗药物 EPI。此外，我们的数据还表明， 蛋白显著影响三种研究的癌细胞系的细胞周期并诱导细胞凋亡；相比之下，它对正常细胞系（即 NIH-3T3、HaCaT 和 16HBE）没有影响。最后，我们的结果还表明， 蛋白在抑制细胞迁移方面表现出优异的性能。总之， 蛋白靶向癌细胞周期并诱导癌细胞凋亡；与 EPI 相比，其抑制癌细胞迁移的优越性表明它具有作为有前途的抗癌药物的潜力。
