从N-[4-(5-硝基-2-呋喃基)-2-噻唑基]甲酰胺诱导的小鼠膀胱癌细胞系中鉴定生长抑制因子
Identification of inhibitory growth factors from a N-[4-(5-nitro-2-furyl)-2-thiazolyl]formamide-induced murine bladder cancer cell line.
作者信息
Heckl W, Jocham K, Täuber J, Vohr H W
机构信息
Department of Urology, Medical School, University of Würzburg, FRG.
出版信息
Urol Int. 1987;42(4):271-6. doi: 10.1159/000281954.
The N-[4-(5-nitro-2-furyl)-2-thiazolyl]formamide (FANFT)-induced murine bladder carcinoma cell line MBT-683 contains growth-stimulating (TGF) and growth-inhibiting factors (GI). These activities coeluted on BioGel P-30 between molecular weights 6,000 and 29,000 daltons. They can be separated by high performance liquid chromatography. Both activities were destroyed by trypsin and dithiothreitol. However, they were resistant to acid and heat. Pool 1 (TGF) stimulated the growth of normal rat kidney cells, whereas pool 2 (GI) inhibited the colony formation of the cell lines MBT-683 and RBTCC-8.
N-[4-(5-硝基-2-呋喃基)-2-噻唑基]甲酰胺（FANFT）诱导的小鼠膀胱癌细胞系MBT-683含有生长刺激因子（TGF）和生长抑制因子（GI）。这些活性物质在分子量6000至29000道尔顿之间于生物凝胶P-30上共洗脱。它们可用高效液相色谱法分离。两种活性均被胰蛋白酶和二硫苏糖醇破坏。然而，它们对酸和热具有抗性。组分1（TGF）刺激正常大鼠肾细胞的生长，而组分2（GI）抑制细胞系MBT-683和RBTCC-8的集落形成。
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