利用脉冲准分子 KrF 激光器对成螨和卵进行紫外线-C 照射的杀螨效果。

Acaricidal efficacy of ultraviolet-C irradiation of Tetranychus urticae adults and eggs using a pulsed krypton fluoride excimer laser.

机构信息

Centre for Applied Molecular Technologies, Institute of Clinical and Experimental Research, Université catholique de Louvain, Tour Claude Bernard, Avenue Hippocrate 54-55, First floor, B1.54.01, 1200, Brussels, Belgium.

LDI Innovation OÜ, Sära 7, Peetri, Estonia.

出版信息

Parasit Vectors. 2021 Nov 17;14(1):578. doi: 10.1186/s13071-021-05085-7.

DOI:10.1186/s13071-021-05085-7

PMID:34789326

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC8596343/

Abstract

BACKGROUND

Pulsed ultraviolet (UV)-C light sources, such as excimer lasers, are used in emerging non-thermal food-decontamination methods and also have high potential for use in a wide range of microbial decontamination applications. The acaricidal effect of an experimental UV-C irradiation device was assessed using female adults and eggs of a model organism, the two-spotted spider mite Tetranychus urticae.

METHODS

UV-C light was generated by a pulsed krypton fluoride excimer laser operating at 248-nm emission wavelength. The pulse energy and pulse repetition rate were 5 mJ and up to 100 Hz, respectively. The distance from the light source to the target was 150 mm; the target surface area was 2.16 cm. The exposure time for the mites and fresh eggs varied from 1 to 4 min at 5-300 mW, which corresponded to UV doses of 5-80 kJ/m. Post-irradiation acaricidal effects (mite mortality) were assessed immediately and also measured at 24 h. The effects of UV-C irradiation on the hatchability of eggs were observed daily for up to 12 days post-irradiation.

RESULTS

The mortality of mites at 5 and 40 kJ/m was 26% and 92%, respectively. Mite mortality reached 98% at 80 kJ/m. The effect of exposure duration on mortality was minimal. The effect of irradiation on egg hatchability was even more significant than that on adult mite mortality, i.e. about 100% egg mortality at an accumulated dose of as little as 5 kJ/m for each exposure time.

CONCLUSIONS

A high rate of mite mortality and lethal egg damage were observed after less than 1 min of exposure to 5 mJ UV-C pulsed irradiation at 60 Hz. Pending further developments (such as beam steering, beam shaping and miniaturisation) and feasibility studies (such as testing with mites in real-life situations), the reported results and characteristics of the UV-C generator (modulation of energy output and adaptability to varying spot sizes) open up the use of this technology for a vast field of acaricidal applications that require long-range radiation.

摘要

背景

脉冲紫外线（UV）-C 光源，如准分子激光器，用于新兴的非热食品消毒方法，也具有广泛用于微生物消毒应用的巨大潜力。使用模式生物二斑叶螨的雌性成虫和卵来评估一种实验性 UV-C 辐照装置的杀螨效果。

方法

使用在 248nm 发射波长下运行的脉冲氟化氪准分子激光器产生 UV-C 光。脉冲能量和脉冲重复率分别为 5mJ 和高达 100Hz。光源到目标的距离为 150mm；目标表面积为 2.16cm。螨虫和新鲜卵的暴露时间从 1 到 4 分钟不等，辐照剂量为 5 到 80kJ/m，辐照器的脉冲重复频率为 5 到 300mW。辐照后立即评估杀螨效果（螨虫死亡率），并在 24 小时后进行测量。辐照后，每天观察卵孵化率，最多达 12 天。

结果

在 5 和 40kJ/m 时，螨虫死亡率分别为 26%和 92%。在 80kJ/m 时，螨虫死亡率达到 98%。暴露时间对死亡率的影响最小。辐照对卵孵化率的影响甚至比成虫螨虫死亡率更为显著，即对于每种暴露时间，只需累积剂量为 5kJ/m，就可达到约 100%的卵死亡率。

结论

在 60Hz 下，每脉冲 5mJ 的 UV-C 脉冲辐照不到 1 分钟，即可观察到螨虫死亡率高和卵致命损伤。在进一步发展（如光束转向、光束整形和小型化）和可行性研究（如在现实情况下用螨虫进行测试）之前，报告的结果和 UV-C 发生器的特性（能量输出的调制和适应不同光斑尺寸的能力）为该技术在需要远程辐射的广泛杀螨应用领域的应用开辟了道路。