Claretiano University Center, Rio Claro, SP, Brazil.
Claretiano University Center, Rio Claro, SP, Brazil.
Photodiagnosis Photodyn Ther. 2021 Mar;33:102161. doi: 10.1016/j.pdpdt.2020.102161. Epub 2020 Dec 26.
Surface decontamination of hospital environments is essential to ensure the safety of health professionals and patients. This process is usually performed through active chemicals substances with high toxicity, and new decontamination technologies that do not leave residues have been currently used, such as UV-C light. Thus, the objective of the present study is to evaluate the effectiveness of a portable UV-C light device on the viability of standard pathogenic strains and other microorganisms isolated from different surfaces of a public health hospital.
In vitro decontamination was performed by applying Biosept Home© UV-C to Staphylococcus aureus, Staphylococcus epidermidis, Escherichia coli, Pseudomonas aeruginosa, Salmonella enterica and Candida albicans. In real conditions, the application was made on different surfaces of a hospital. The device used in the experiment haa a 254 nm UV-C light and a radiation intensity of 45.6 mW/cm over a distance of 1 cm from the surfaces. The light dose was 0.912 J/cm for 20 s of application in both conditions (in vitro and hospital).
After in vitro decontamination with UV-C light no bacterial growth was observed, demonstrating 100 % of bacterial inactivation under the conditions tested. Additionally, there was a reduction of approximately 4 logs for the yeast C. albicans. In all hospital surfaces, the number of colonies of microorganisms was significantly reduced after the procedure.
The results suggest that Biosept Home© UV-C is efficient and constitutes a promosing intervention for disinfection protocols in hospitals and clinics.
医院环境的表面去污对于确保卫生专业人员和患者的安全至关重要。这一过程通常通过具有高毒性的活性化学物质来完成，目前已经使用了不会留下残留物的新的去污技术，例如 UV-C 光。因此，本研究的目的是评估一种便携式 UV-C 光设备对从公共卫生医院不同表面分离出的标准致病菌株和其他微生物的生存能力的有效性。
通过将 Biosept Home©UV-C 应用于金黄色葡萄球菌、表皮葡萄球菌、大肠杆菌、铜绿假单胞菌、肠炎沙门氏菌和白色念珠菌来进行体外去污。在真实条件下，将该设备应用于医院的不同表面。该实验中使用的设备在距离表面 1 厘米处发出 254nm 的 UV-C 光，辐射强度为 45.6mW/cm。在两种条件（体外和医院）下，应用 20 秒的光剂量为 0.912J/cm。
经 UV-C 光体外去污后，未观察到细菌生长，表明在测试条件下细菌灭活率达到 100%。此外，酵母白色念珠菌的数量减少了约 4 个对数级。在所有医院表面，处理后微生物的菌落数量均显著减少。
结果表明，Biosept Home©UV-C 高效且有望成为医院和诊所消毒方案的一种干预措施。
