Li Xiaofei, Chen Shujia, Zhu Yinghui, Fei Jiayue, Song Liaoyuan, Sun Guoyan, Niu Wei, Guo Lianyi, Wang Jiwei
Department of Gastroenterology, The First Affiliated Hospital of Jinzhou Medical University, Jinzhou, China.
Department of Gastrointestinal Surgery, Xuzhou Central Hospital, Xuzhou, China.
J Gastrointest Oncol. 2021 Oct;12(5):2132-2149. doi: 10.21037/jgo-21-598.
The Homeobox B () family promotes tumor progression, but the mechanism of its action in gastric cancer (GC) is unclear. We sought to identify the family members that are critical to the prognosis of GC patients.
The Oncomine, Gene Expression Profiling Interactive Analysis (GEPIA), cBioPortal, UALCAN, Kaplan-Meier plotter, and the GeneMANIA databases were used to analyze the messenger RNA (mRNA) expression levels, prognostic value, and gene-gene interaction network of the family members in GC. The expression of in GC and its relationship with various clinicopathological parameters and the prognosis of patients were verified by immunohistochemistry.
The expression of , , and mRNA was significantly upregulated in GC. There was a significant correlation between the upregulation of , and mRNA and a low overall survival (OS) rate. The high expression of , , and mRNA was closely correlated to tumor grade and stage. was the family member most closely related to the occurrence and development of GC. A further analysis showed that might be involved in deoxyribonucleic acid repair and division regulation. A validation study showed that the advanced cancer group had a higher level of expression than the early cancer group. The high expression of in gastric tissue plays an important role in the survival and prognosis of GC patients.
HOXB family members have different degrees of abnormal expression in GC. High expression in GC tissues was significantly correlated with a worse prognosis. Thus, is a potential novel biomarker and therapeutic target for GC.
同源盒B（HOXB）家族促进肿瘤进展，但其在胃癌（GC）中的作用机制尚不清楚。我们试图确定对GC患者预后至关重要的HOXB家族成员。
使用Oncomine、基因表达谱交互分析（GEPIA）、cBioPortal、UALCAN、Kaplan-Meier绘图仪和GeneMANIA数据库分析HOXB家族成员在GC中的信使核糖核酸（mRNA）表达水平、预后价值和基因-基因相互作用网络。通过免疫组织化学验证HOXB在GC中的表达及其与各种临床病理参数和患者预后的关系。
HOXB5、HOXB7和HOXB9的mRNA在GC中表达显著上调。HOXB5、HOXB7和HOXB9的mRNA上调与低总生存率（OS）之间存在显著相关性。HOXB5、HOXB7和HOXB9的mRNA高表达与肿瘤分级和分期密切相关。HOXB9是与GC发生发展最密切相关的HOXB家族成员。进一步分析表明，HOXB9可能参与脱氧核糖核酸修复和分裂调控。一项验证研究表明，晚期癌症组的HOXB9表达水平高于早期癌症组。胃组织中HOXB9的高表达在GC患者的生存和预后中起重要作用。
HOXB家族成员在GC中存在不同程度的异常表达。GC组织中HOXB9的高表达与较差的预后显著相关。因此，HOXB9是GC潜在的新型生物标志物和治疗靶点。
