1 Department of General Surgery, Second Affiliated Hospital of Nanchang University, Nanchang 330006, China ; 2 Department of Gastrointestinal Surgery, Jiangxi Provincial Cancer Hospital, Nanchang 330029, China ; 3 Jiangxi Province Key Laboratory of Molecular Medicine, Nanchang 330006, China.
Chin J Cancer Res. 2014 Feb;26(1):72-80. doi: 10.3978/j.issn.1000-9604.2014.01.11.
Homeobox B9 (HOXB9) is proposed to be involved in tumor angiogenesis and metastasis. We investigated the role of HOXB9 in the progression of colon cancer.
HOXB9 expression was investigated by immunohistochemically and Western blotting in 128 colon cancer patients and the results were analyzed statistically associated with clinicopathological data and survival of the patients. The effect of HOXB9 on cell invasion and metastases abilities were analyzed in vitro and in vivo.
HOXB9 is overexpressed in colon cancer tissues and significantly correlated with metastasis and poor survival of patients (P<0.05, respectively). Additionally, high levels of expression of HOXB9 were observed in metastatic lymph nodes. The down-regulation of HOXB9 expression can inhibit the migration and invasive ability of colon cancer cells, while exogenous expression of HOXB9 in colon cancer cells enhanced cell migration and invasiveness. Moreover, stable knockdown of HOXB9 reduced the liver and lung metastasis of colon cancer in vivo.
HOXB9 may play an important role in the invasion and metastasis of colon cancer cells and may be a useful biomarker for metastasis and prognostic of colon cancer.
同源盒基因 B9（HOXB9）被认为参与肿瘤血管生成和转移。本研究旨在探讨 HOXB9 在结肠癌进展中的作用。
采用免疫组织化学和 Western blot 法检测 128 例结肠癌患者中 HOXB9 的表达，并对其与患者临床病理数据和生存的关系进行统计学分析。在体外和体内分析 HOXB9 对细胞侵袭和转移能力的影响。
HOXB9 在结肠癌组织中过表达，与转移和患者预后不良显著相关（P<0.05）。此外，HOXB9 的高表达也见于转移性淋巴结。下调 HOXB9 的表达可抑制结肠癌细胞的迁移和侵袭能力，而外源性表达 HOXB9 可增强结肠癌细胞的迁移和侵袭能力。此外，稳定敲低 HOXB9 可减少结肠癌在体内的肝和肺转移。
HOXB9 可能在结肠癌细胞的侵袭和转移中发挥重要作用，可作为结肠癌转移和预后的有用标志物。
