Sugahara K, Okamoto H, Nakamura M, Shibamoto S, Yamashina I
Department of Biological Chemistry, Faculty of Pharmaceutical Sciences, Kyoto University, Japan.
Arch Biochem Biophys. 1987 Nov 1;258(2):391-403. doi: 10.1016/0003-9861(87)90360-2.
Glycosaminoglycan sulfotransferase activities in sera during the prenatal and postnatal development of the ox, rat, and chicken were systematically measured with chemically desulfated cartilage chondroitin 4-sulfate, cornea keratan sulfate, and kidney heparan sulfate as exogenous sulfate acceptors and with [35S]sulfate-labeled 3'-phosphoadenosine 5'-phosphosulfate as a sulfate donor. The results of specificity studies and product analyses indicated that these enzymes introduce sulfates at position 6 of the internal N-acetylgalactosamine units of chondroitin, position 6 of the galactose units of keratan sulfate, and position 2 (an amino group) of the glucosamine units of heparan sulfate, respectively. The results of the enzyme assays indicated that (1) the three activities change in a development-associated manner in each animal species, (2) generally, the activities of the former two enzymes decrease with embryonic development and aging after birth, although in chicken serum they increase transiently at the late prenatal stage and decrease thereafter, and (3) the pattern of the changes in heparan sulfate sulfotransferase activity is species-dependent: the activity increases in the rat, decreases in the ox, and does not significantly change in the chicken during prenatal or postnatal development. These alterations may reflect development-associated biosynthesis of the corresponding glycosaminoglycans or maturation of the proteoglycans in some tissues.
以化学脱硫的软骨硫酸软骨素4 - 硫酸酯、角膜硫酸角质素和肾硫酸乙酰肝素作为外源性硫酸受体，并以[35S]硫酸标记的3'-磷酸腺苷5'-磷酸硫酸作为硫酸供体，系统地测定了牛、大鼠和鸡在产前和产后发育过程中血清中的糖胺聚糖硫酸转移酶活性。特异性研究和产物分析结果表明，这些酶分别在硫酸软骨素内部N - 乙酰半乳糖胺单元的6位、硫酸角质素半乳糖单元的6位和硫酸乙酰肝素葡糖胺单元的2位（氨基）引入硫酸根。酶活性测定结果表明：(1) 每种动物的这三种活性都以与发育相关的方式变化；(2) 一般来说，前两种酶的活性随着胚胎发育和出生后的衰老而降低，尽管在鸡血清中它们在产前后期短暂升高，之后降低；(3) 硫酸乙酰肝素硫酸转移酶活性的变化模式因物种而异：在大鼠中活性增加，在牛中降低，在鸡的产前或产后发育过程中没有显著变化。这些变化可能反映了相应糖胺聚糖的发育相关生物合成或某些组织中蛋白聚糖的成熟。
