Sugahara K, Shibamoto S, Yamashina I
FEBS Lett. 1985 Apr 8;183(1):43-6. doi: 10.1016/0014-5793(85)80950-9.
Assay conditions for chondroitin sulfotransferase in serum were established using a rapid and simple paper disk method. Sulfotransferase activities towards endogenous serum glycosaminoglycans and exogenously added chondroitin were determined for fetal, newborn and adult bovine sera. The results indicate that 3'-phosphoadenosine 5'-phosphosulfate (PAPS); chondroitin 6-sulfotransferase activity in fetal calf serum is several times that in newborn or adult bovine sera. The enzyme transfers sulfate onto position 6 of internal nonsulfated galactosamine units of endogenous and exogenous chondroitin.
采用快速简便的纸片法建立了血清中硫酸软骨素磺基转移酶的检测条件。测定了胎牛、新生牛和成年牛血清对内源性血清糖胺聚糖和外源性添加的软骨素的磺基转移酶活性。结果表明，3'-磷酸腺苷5'-磷酸硫酸酯（PAPS）；胎牛血清中的软骨素6-磺基转移酶活性是新生牛或成年牛血清中的几倍。该酶将硫酸基团转移到内源性和外源性软骨素内部非硫酸化半乳糖胺单元的6位上。
