Wu Zhiqiang, Li Jianqi, Chen Ludan, Chen Song, Zhuang Wenquan
Department of Interventional Radiology, The First Affiliated Hospital of Sun Yat-sen University, Guangzhou, China.
Department of Radiology, The First Affiliated Hospital of Sun Yat-sen University, Guangzhou, China.
Ann Transl Med. 2021 Oct;9(20):1528. doi: 10.21037/atm-21-4297.
The aim of the present study was to construct an model of degenerated nucleus pulposus with different combinations of biochemical components, and to find an model for the early degeneration of nucleus pulposus suitable for the detection of magnetic resonance T1rho (MR-T1ρ) sequence for the early diagnosis of degeneration of lumbar intervertebral disc.
The proteoglycan concentration gradient in the first experimental group was 5%, with a concentration range of 7 samples models from 5% to 35%. The second experimental group had 15 samples with a 1% concentration gradient of proteoglycan (range, 10-24%), with a higher water content compared with the first group. The third experimental group contained 20 samples with a concentration gradient of 1% proteoglycan (range, 10-29%), with 75% water content. All of the models were scanned using a 3.0T GE MR. To analyze the correlation between the proteoglycan content of the model and the T1ρ value, we investigated the feasibility and stability of modeling.
There was no correlation between the model proteoglycan concentration and T1ρ value in the first experimental group; however, there was a significant negative correlation between the proteoglycan concentration and T1ρ value in the second experimental group (Y=-3.02X+131.8, R=0.852, P<0.05). In the third experimental group, the proteoglycan concentration was significantly positively correlated with T1ρ value (Y=3.05X+11.99, R=0.834, P<0.05). The comparison of the T1ρ values in the third experimental group before and 3 months after yielded an intraclass correlation coefficient value of 0.980, indicating that the biochemical components in the third experimental group were still stable after 3 months of storage. The slope of the regression equation between the Pfirrmann grading and T1ρ value in the third experimental group was not statistically different from the volunteer group (F=0.54, P=0.814), suggesting that the lumbar disc nucleus pulposus tissue of model samples fitted well with the volunteer group.
In this experiment, we successfully constructed an model of nucleus pulposus tissue proteoglycan that can be used for the quantitative evaluation of the MR-T1ρ imaging.
本研究旨在构建具有不同生化成分组合的退变髓核模型，并找到一种适合检测磁共振T1rho（MR-T1ρ）序列以早期诊断腰椎间盘退变的髓核早期退变模型。
第一实验组的蛋白聚糖浓度梯度为5%，7个样本模型的浓度范围为5%至35%。第二实验组有15个样本，蛋白聚糖浓度梯度为1%（范围为10%-24%），与第一组相比含水量更高。第三实验组包含20个样本，蛋白聚糖浓度梯度为1%（范围为10%-29%），含水量为75%。所有模型均使用3.0T GE MR进行扫描。为分析模型的蛋白聚糖含量与T1ρ值之间的相关性，我们研究了建模的可行性和稳定性。
第一实验组模型的蛋白聚糖浓度与T1ρ值之间无相关性；然而，第二实验组的蛋白聚糖浓度与T1ρ值之间存在显著负相关（Y=-3.02X+131.8，R=0.852，P<0.05）。在第三实验组中，蛋白聚糖浓度与T1ρ值呈显著正相关（Y=3.05X+11.99，R=0.834，P<0.05）。第三实验组在储存3个月前后的T1ρ值比较得出组内相关系数值为0.980，表明第三实验组的生化成分在储存3个月后仍保持稳定。第三实验组中Pfirrmann分级与T1ρ值之间回归方程的斜率与志愿者组无统计学差异（F=0.54，P=0.814），表明模型样本的腰椎间盘髓核组织与志愿者组拟合良好。
在本实验中，我们成功构建了一种可用于MR-T1ρ成像定量评估的髓核组织蛋白聚糖模型。
