Embery G, Picton D C, Stanbury J B
Department of Dental Sciences, University of Liverpool, England, U.K.
Arch Oral Biol. 1987;32(8):545-9. doi: 10.1016/0003-9969(87)90062-8.
Proteoglycan-like fractions (PG) were isolated from the ligaments of teeth undergoing various degrees of intrusive loadings. The PG were characterized by their molecular-size profiles on Sepharose 4B, the presence of uronic acid in the separated fractions and by the electrophoretic detection of constituent glycosaminoglycans including heparan sulphate, hyaluronic acid, dermatan sulphate and chondroitin-4-sulphate. The high molecular-weight fraction, peak i (estimated minimum size, 2 X 10(6) daltons) of the normal-functioning (stressed) ligament was reduced approx. 70 per cent, compared with ligament undisturbed for 3 h. There was a decrease in peak-i size between 0.25 and 1 N loadings of approx. 72 per cent. The 4 N loadings produced a further decrease followed by an increase during a 3 h undisturbed recovery phase. Thus changes in the chemistry and properties of the ground-substance components of the periodontal ligament could partly explain changes in tooth mobility.
从承受不同程度侵入性负荷的牙齿韧带中分离出类蛋白聚糖组分（PG）。通过PG在琼脂糖4B上的分子大小分布、分离组分中糖醛酸的存在以及对包括硫酸乙酰肝素、透明质酸、硫酸皮肤素和硫酸软骨素-4在内的组成糖胺聚糖的电泳检测来对PG进行表征。与未受干扰3小时的韧带相比，正常功能（受力）韧带的高分子量组分，即峰i（估计最小尺寸为2×10⁶道尔顿）减少了约70%。在0.25至1牛顿负荷之间，峰i的尺寸减少了约72%。4牛顿负荷导致进一步减少，随后在3小时的未受干扰恢复阶段出现增加。因此，牙周韧带基质成分的化学和性质变化可以部分解释牙齿移动性的变化。
