Laboratory of Organic Chemistry, Wageningen University & Research, Stippeneng 4, 6807 WE, Wageningen, The Netherlands.
Chem Commun (Camb). 2021 Dec 3;57(96):12960-12963. doi: 10.1039/d1cc05446e.
Two acyl-transfer catalysts were conjugated to thrombin-binding DNA aptamers to acylate thrombin. Modification occurred site-selectively on Lys (≫Ser) residues proximal to the respective aptamer-thrombin interface, was selective for thrombin in the presence of other proteins, and the activity of both DNA-catalysts could be controlled by an external trigger.
两个酰基转移催化剂被连接到凝血酶结合 DNA 适体上来酰化凝血酶。修饰选择性地发生在靠近各自适体-凝血酶界面的 Lys（≫Ser）残基上，在存在其他蛋白质的情况下对凝血酶具有选择性，并且两个 DNA 催化剂的活性都可以被外部触发控制。
