Laboratory of Organic Chemistry, Wageningen University and Research, Wageningen, The Netherlands.
Biopolymers. 2022 Mar;113(3):e23483. doi: 10.1002/bip.23483. Epub 2021 Dec 8.
Protein modification is important for various types of biomedical research, including proteomics and therapeutics. Many methodologies for protein modification exist, but not all possess the required level of efficiency and site selectivity. This review focuses on the use of DNA to achieve the desired conversions and levels of accuracy in protein modification by using DNA (i) as a template to help concentrate dilute reactants, (ii) as a guidance system to achieve selectivity by binding specific proteins, and (iii) even as catalytic entity or construct to enhance protein modification reactions.
蛋白质修饰对于包括蛋白质组学和治疗学在内的各种类型的生物医学研究都很重要。有许多蛋白质修饰的方法，但并非所有方法都具有所需的效率和位点选择性。本文综述了利用 DNA 作为模板来浓缩稀释反应物、作为结合特定蛋白质以实现选择性的指导系统，甚至作为催化实体或构建体来增强蛋白质修饰反应的方法，实现蛋白质修饰所需的转化和准确性。
