State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing, China.
Department of Entomology, Faculty of Agriculture, University of Agriculture Faisalabad, Faisalabad, Pakistan.
Pest Manag Sci. 2022 Sep;78(9):3903-3911. doi: 10.1002/ps.6725. Epub 2021 Dec 6.
The potato tuber moth, Phthorimaea operculella is a key pest of potato and tobacco in China. We compared the sex pheromone titers released by P. operculella female, and the electroantennogram (EAG) responses of male antennae to pheromone compounds under laboratory conditions. Then, the optimal sex pheromone ratio was screened in the field.
The P. operculella sex pheromone feeding on potato or tobacco was extracted by solvent-extraction method. Main sex pheromone compounds including E4, Z7-13: AC (PTM1) and E4, Z7, Z10-13: AC (PTM2) were found in gland. The titer and relative ratio of P. operculella sex pheromone compounds secreted by female adult moths differ between host populations, while the relative EAG responses trend of P. operculella males to the pheromone components were the same when directly stimulated. In field trials, PTM1:PTM2 = 8:1-4:1 and PTM1:PTM2 = 1:4-1:7 was the most attractive ratio to P. operculella males in tobacco and potato fields, respectively. During 2019-2020, after 8 weeks of mass trapping for each year, the sex pheromone could reduce the population of P. operculella in the potato and tobacco fields.
In this study, fixed property and quantity analyzing methods were adopted to compare sex pheromones from feeding on potato versus tobacco. EAG tests then were carried out on P. operculella males. Finally, we optimized the ratio of two sex pheromone compounds in potato and examined how this influenced field trapping. By further improving the parameters of sex pheromone application in the field, we demonstrate that deployment of sex pheromones can provide effective control of P. operculella. © 2021 Society of Chemical Industry.
马铃薯块茎蛾，Phthorimaea operculella 是中国马铃薯和烟草的主要害虫。我们比较了实验室条件下马铃薯块茎蛾雌虫释放的性信息素量和雄虫触角对性信息素化合物的电生理反应（EAG），然后在田间筛选出最佳性信息素比例。
从取食马铃薯或烟草的马铃薯块茎蛾中用溶剂萃取法提取性信息素。在腺体中发现了主要的性信息素化合物包括 E4、Z7-13：AC（PTM1）和 E4、Z7、Z10-13：AC（PTM2）。不同寄主种群的成年雌蛾分泌的性信息素化合物的含量和相对比例不同，而当直接刺激时，雄蛾对性信息素成分的相对 EAG 反应趋势是相同的。在田间试验中，PTM1：PTM2=8：1-4：1 和 PTM1：PTM2=1：4-1：7 分别是烟草和马铃薯田中最吸引马铃薯块茎蛾雄虫的比例。在 2019-2020 年，每年进行 8 周的大量诱捕后，性信息素可以降低马铃薯和烟草田中马铃薯块茎蛾的种群数量。
本研究采用固定属性和定量分析方法比较了取食马铃薯和烟草的性信息素，然后对马铃薯块茎蛾雄虫进行了 EAG 测试，最后优化了两种性信息素化合物在马铃薯中的比例，并研究了这对田间诱捕的影响。通过进一步改进田间性信息素应用的参数，我们证明了性信息素的释放可以有效控制马铃薯块茎蛾。
