Gerwin B I, Lechner J F, Reddel R R, Roberts A B, Robbins K C, Gabrielson E W, Harris C C
Laboratory of Human Carcinogenesis, National Cancer Institute, Bethesda, Maryland 20892.
Cancer Res. 1987 Dec 1;47(23):6180-4.
Steady state mRNA levels for transforming growth factor beta, platelet-derived growth factor (PDGF) A-chain, and PDGF B-chain were measured in normal human mesothelial cells, SV40 large T-antigen expressing human mesothelial cells, and human mesothelioma cell lines. The mRNA expression level for transforming growth factor beta was similar in all three types of culture while normal human mesothelial cells secrete more transforming growth factor beta than do mesothelioma cell lines or T-antigen expressing mesothelial cells. In contrast, both PDGF A- and B-chain mRNAs are expressed at higher levels in mesothelioma cell lines than in normal human mesothelial cells. PDGF-like mitogenic activity was readily detectable in medium conditioned by a mesothelioma cell line and was undetectable in conditioned medium from normal cells. These results suggest the hypothesis that PDGF may be an autocrine growth factor in mesothelioma.
在正常人腹膜间皮细胞、表达SV40大T抗原的人腹膜间皮细胞以及人恶性间皮瘤细胞系中，检测了转化生长因子β、血小板衍生生长因子（PDGF）A链和PDGF B链的稳态mRNA水平。在所有三种培养类型中，转化生长因子β的mRNA表达水平相似，而正常人腹膜间皮细胞分泌的转化生长因子β比恶性间皮瘤细胞系或表达T抗原的腹膜间皮细胞更多。相比之下，PDGF A链和B链的mRNA在恶性间皮瘤细胞系中的表达水平均高于正常人腹膜间皮细胞。在恶性间皮瘤细胞系条件培养基中很容易检测到PDGF样促有丝分裂活性，而在正常细胞的条件培养基中则检测不到。这些结果提示了一个假说，即PDGF可能是恶性间皮瘤中的一种自分泌生长因子。
