Engineering Research Center of Industrial Microbiology of Ministry of Education, College of Life Sciences, Fujian Normal University, Fuzhou, Fujian 350117, China.
Center of Engineering Technology Research for Microalgae Germplasm Improvement of Fujian, Southern Institute of Oceanography, College of Life Sciences, Fujian Normal University, Fuzhou, Fujian 350117, China.
Langmuir. 2021 Dec 7;37(48):14147-14156. doi: 10.1021/acs.langmuir.1c02389. Epub 2021 Nov 18.
The opal-like SiO microcarriers with different pore diameters named opal-SiOI and opal-SiOII were synthesized and utilized as microcarriers to immobilize lipase (ROL) and α-amylases (AOA). ROL and AOA can be more stably immobilized on the cross-linked SiO opals by neopentyl glycol diglycidyl ether (NGDE), which is the first attempt to use it as a cross-linking agent compared with glutaraldehyde. According to the morphology analysis, multiple layers of SiO monodisperse microspheres were regularly packed and formed an opal-like structure, and enzymes were well scattered and immobilized throughout the SiO opals. The results showed that the performance of enzymes immobilized on opal-SiOII with a larger specific surface area was much better than that of opal-SiOI. The enzyme activity of ROL@opal-SiOII and AOA@opal-SiOII cross-linked with 1% NGDE increased 5.32 and 9.32 times compared with their free counterpart, respectively. Furthermore, pH and thermal stability and reusability of ROL/AOA@opal-SiOII were significantly improved and higher than those of ROL/AOA@opal-SiOI and free enzymes. This study provides an easily obtained microcarrier opal-SiOII, which shows potential for efficient different enzyme immobilizations and further industrial applications.
具有不同孔径的蛋白石状 SiO 微载体，分别命名为蛋白石-SiOI 和蛋白石-SiOII，被合成并用作固定化脂肪酶 (ROL) 和 α-淀粉酶 (AOA) 的微载体。ROL 和 AOA 可以通过新戊二醇二缩水甘油醚 (NGDE) 更稳定地固定在交联的 SiO 蛋白石上，与戊二醛相比，这是首次尝试将其用作交联剂。根据形态分析，多层 SiO 单分散微球规则排列并形成蛋白石状结构，酶均匀分散并固定在整个 SiO 蛋白石中。结果表明，比表面积较大的蛋白石-SiOII 固定化酶的性能要好得多。与游离酶相比，用 1% NGDE 交联的 ROL@opal-SiOII 和 AOA@opal-SiOII 的酶活性分别提高了 5.32 倍和 9.32 倍。此外，ROL/AOA@opal-SiOII 的 pH 和热稳定性以及可重复使用性得到了显著提高，高于 ROL/AOA@opal-SiOI 和游离酶。这项研究提供了一种易于获得的微载体蛋白石-SiOII，它显示出在高效固定化不同酶方面的潜力，并具有进一步的工业应用前景。
