The Key Laboratory of Synthetic and Biological Colloids, Ministry of Education, School of Chemical and Material Engineering, Jiangnan University, Wuxi, 214122, People's Republic of China.
Appl Biochem Biotechnol. 2023 Nov;195(11):6942-6958. doi: 10.1007/s12010-023-04477-y. Epub 2023 Mar 23.
Immobilized lipase is a green and sustainable catalyst for hydrolysis of acidified oil. Glutaraldehyde is widely used for lipase immobilization while the appropriate strategy optimizes the catalytic performance of lipase. In this research, lipase from Candida rugosa (CRL) was immobilized on spherical silica (SiO) by glutaraldehyde multipoint covalent treatments, including covalent binding method and adsorption-crosslinking method. The enzymatic stability properties and performance in hydrolysis of refined oil and acidified oil were studied. We confirmed that the residual activity decreased while the stability increased because of the influence on secondary structure of lipase after multipoint covalent treatments. In the comparison of different immobilization strategies in multipoint covalent treatment, SiO-CRL (covalent binding method) showed lower loading capacity than SiO-CRL (adsorption-crosslinking method), resulting in low activity. However, SiO-CRL (covalent binding method) showed better reusability and stability. Immobilized lipase via covalent binding method was more potential in the application of catalytic hydrolysis of acidified oils.
固定化脂肪酶是一种绿色可持续的酸解油脂水解催化剂。戊二醛广泛用于脂肪酶固定化，而适当的策略可以优化脂肪酶的催化性能。在这项研究中，来自 Candida rugosa（CRL）的脂肪酶通过戊二醛多点共价处理固定在球形二氧化硅（SiO）上，包括共价结合法和吸附交联法。研究了酶的稳定性特性以及在精炼油和酸化油水解中的性能。我们证实，由于多点共价处理后脂肪酶二级结构受到影响，残余活性降低，稳定性增加。在多点共价处理中不同固定化策略的比较中，SiO-CRL（共价结合法）的载量比 SiO-CRL（吸附交联法）低，导致活性低。然而，SiO-CRL（共价结合法）表现出更好的可重复使用性和稳定性。通过共价结合法固定化的脂肪酶在催化水解酸化油方面更具应用潜力。
