University of Pardubice, Faculty of Chemical Technology, Department of Analytical Chemistry, Studentská 573, 53210 Pardubice, Czech Republic.
Palacký University Olomouc, Faculty of Medicine and Dentistry, Hněvotínská 5, 77900 Olomouc, Czech Republic.
Biochim Biophys Acta Mol Cell Biol Lipids. 2022 Feb;1867(2):159082. doi: 10.1016/j.bbalip.2021.159082. Epub 2021 Nov 15.
Lung cancer represents one of the leading worldwide causes of cancer death, but the pathobiochemistry of this disease is still not fully understood. Here we characterize the lipidomic and metabolomic profiles of the tumor and surrounding normal tissues for 23 patients with non-small cell lung cancer. In total, 500 molecular species were identified and quantified by a combination of the lipidomic shotgun tandem mass spectrometry (MS/MS) analysis and the targeted metabolomic approach using liquid chromatography (LC) - MS/MS. The statistical evaluation includes multivariate and univariate methods with the emphasis on paired statistical approaches. Our research revealed significant changes in several biochemical pathways related to the central carbon metabolism, acylcarnitines, dipeptides as well as the disruption in the lipid metabolism observed mainly for glycerophospholipids, sphingolipids, and cholesteryl esters.
肺癌是全球主要的癌症死因之一，但该疾病的病理生物化学仍未被完全理解。在这里，我们对 23 名非小细胞肺癌患者的肿瘤和周围正常组织进行了脂质组学和代谢组学特征分析。我们采用脂质组学 shotgun 串联质谱（MS/MS）分析和液相色谱（LC）-MS/MS 靶向代谢组学方法相结合，共鉴定和定量了 500 种分子物种。统计评估包括多元和单变量方法，重点是配对统计方法。我们的研究揭示了与中心碳代谢、酰基辅酶 A、二肽相关的几个生化途径以及脂质代谢中断的显著变化，主要表现在甘油磷脂、鞘脂和胆固醇酯上。
