Erzincan Binali Yildirim University, Medical Faculty, Obstetrics and Gynaecology Department, Erzincan, Turkey.
Ankara Yildirim Beyazit University, Department of Medical Biochemistry, Ankara, Turkey.
Taiwan J Obstet Gynecol. 2021 Nov;60(6):1038-1042. doi: 10.1016/j.tjog.2021.09.015.
Oligohydramnios is defined as amniotic fluid index in ultrasonographic measurement is less than 5 percentile according to gestational age, the amniotic fluid volume is ≤ 5 cm, or if the single deepest dial is < 2 cm. The condition of oligohydramnios that not with fetal structural/chromosomal abnormalities, intrauterine growth retardation, intrauterine infection and maternal disease is described as isolated oligohydramnios (IO). The aim of this study is to examine whether oxidative stress and reactive oxygen species (ROS) have a place in the pathophysiology of IO.
In this prospective case-control study, a total of 126 participants were included. The patient group consisted of 65 patients who were diagnosed IO, and the control group consisted of 61 healthy normal pregnants. Native thiol (-SH), total thiol (-SH + -SS), dynamic disulfide (-SS), IMA values from maternal serum were measured and compared between groups.
Maternal serum -SH and -SH + -SS values were significantly lower in the IO group than in the control group (409.47 ± 55.36 μmol/L vs. 437.40 ± 48.68 μmol/L, p = 0.03 and 457.40 ± 63.01 μmol/L vs. 484.59 ± 52.75 μmol/L, p = 0.01). In the IO group when -SS/-SH and -SS/-SH + -SS ratio was found to be statistically significantly higher than control group (5.84 ± 1.1 vs 5.41 ± 0.71, p = 0.01 and 5.2 ± 0.88 vs 4.8 ± 0.58, p = 0.01), -SH/-SH + -SS ratio was significantly lower (89.56 ± 1.7 vs 90.24 ± 1.16, p = 0.01). There was no significant difference in terms of -SS value (p = 0.66). IMA value was significantly higher in the IO group than control group (0.76 ± 0.10 ABSU vs 0.68 ± 0.06, p < 0.01). It is seen as a result of ROC analysis that -SH, -SH + -SS, -SS/-SH, -SS/-SH + -SS, -SH/-SH + -SS and IMA values have a diagnostic value for IO (p < 0.05).
The thiol/disulfide balance shifted towards oxidative stress in IO compared to control group. So oxidative stress and ROS have a place in the pathophysiology of IO.
羊水过少是指根据妊娠年龄超声测量的羊水指数小于第 5 百分位，羊水体积≤5cm，或单个最深羊水深度<2cm。如果没有胎儿结构/染色体异常、宫内生长受限、宫内感染和母体疾病，则将羊水过少描述为孤立性羊水过少（IO）。本研究旨在探讨氧化应激和活性氧（ROS）是否在 IO 的病理生理学中发挥作用。
在这项前瞻性病例对照研究中，共纳入了 126 名参与者。患者组由 65 名被诊断为 IO 的患者组成，对照组由 61 名健康正常孕妇组成。测量并比较了母体血清中的天然巯基（-SH）、总巯基（-SH+ -SS）、动态二硫键（-SS）、IMA 值。
IO 组母体血清 -SH 和 -SH+ -SS 值明显低于对照组（409.47±55.36μmol/L 与 437.40±48.68μmol/L，p=0.03 和 457.40±63.01μmol/L 与 484.59±52.75μmol/L，p=0.01）。在 IO 组中，-SS/-SH 和 -SS/-SH+ -SS 比值明显高于对照组（5.84±1.1 与 5.41±0.71，p=0.01 和 5.2±0.88 与 4.8±0.58，p=0.01），-SH/-SH+ -SS 比值明显较低（89.56±1.7 与 90.24±1.16，p=0.01）。-SS 值无显著差异（p=0.66）。IO 组的 IMA 值明显高于对照组（0.76±0.10 ABSU 与 0.68±0.06，p<0.01）。ROC 分析结果表明，-SH、-SH+ -SS、-SS/-SH、-SS/-SH+ -SS、-SH/-SH+ -SS 和 IMA 值对 IO 具有诊断价值（p<0.05）。
与对照组相比，IO 中巯基/二硫键平衡向氧化应激倾斜。因此，氧化应激和 ROS 在 IO 的病理生理学中发挥作用。
