Katar-Yildirim Ceren, Tokmak Aytekin, Yildirim Cagdas, Erel Ozcan, Caglar Ali Turhan
a Department of Obstetrics and Gynecology , Zekai Tahir Burak Women?s Health Research and Education Hospital , Ankara , Turkey.
b Department of Emergency Medicine , Ataturk Training and Research Hospital , Ankara , Turkey.
J Matern Fetal Neonatal Med. 2018 Sep;31(18):2457-2462. doi: 10.1080/14767058.2017.1344962. Epub 2017 Jul 4.
The aim of our study is to compare serum thiol/disulphide homeostasis of women diagnosed with abortus imminens (AI) and healthy pregnant women, and to determine whether it has a role in the pathogenesis of the disease or not.
A total of 100 pregnant women were included in the study. The study group consisted of 50 patients with AI whereas 50 healthy pregnant women were chosen as the control group. All of the patients in the two groups were matched for age, gestational age, and body mass index. Thiol/disulphide levels were analyzed with a newly developed automated spectrophotometric method.
We found significantly reduced levels of native thiol (SH) (370.2 ± 35.2 μmol/l versus 397.6 ± 29.3 μmol/l) and total thiol (406 ± 37.1 μmol/l versus 434.5 ± 29.9 μmol/l), in the sera of the study group compared to the control group (p < .001). But serum levels of disulphide (SS) were statistically insignificant between the two groups (17.9 ± 5.9 μmol/l versus 18.4 ± 4.9 μmol/l, p: .612). Similarly, no significant differences were observed between the two groups in terms of SS/SH, SS/total thiol and SH/total thiol ratios (all p > .05).
We found a decrease in thiol levels which have antioxidant properties in patients with AI. Decreased antioxidant level in the body is thought to play a role in the etiology of AI. In this regard, further studies are needed to elucidate the potential role of dynamic thiol/disulphide homeostasis in the pathogenesis of AI.
本研究旨在比较诊断为难免流产（AI）的女性与健康孕妇的血清硫醇/二硫化物稳态，并确定其是否在该疾病的发病机制中起作用。
本研究共纳入100名孕妇。研究组由50例难免流产患者组成，同时选取50名健康孕妇作为对照组。两组所有患者在年龄、孕周和体重指数方面均匹配。采用新开发的自动分光光度法分析硫醇/二硫化物水平。
我们发现，与对照组相比，研究组血清中天然硫醇（SH）水平显著降低（370.2±35.2μmol/l对397.6±29.3μmol/l），总硫醇水平也显著降低（406±37.1μmol/l对434.5±29.9μmol/l）（p＜0.001）。但两组间二硫化物（SS）的血清水平无统计学差异（17.9±5.9μmol/l对18.4±4.9μmol/l，p：0.612）。同样，两组在SS/SH、SS/总硫醇和SH/总硫醇比值方面也未观察到显著差异（所有p＞0.05）。
我们发现难免流产患者体内具有抗氧化特性的硫醇水平降低。体内抗氧化水平降低被认为在难免流产的病因中起作用。在这方面，需要进一步研究以阐明动态硫醇/二硫化物稳态在难免流产发病机制中的潜在作用。
