Rousset H, Chanteloube P, Magistrini M, Palmer E
Institut du Cheval, Arnac-Pompadour, France.
J Reprod Fertil Suppl. 1987;35:25-31.
(1) Various estimations of motility (subjective appreciation, count on video record, Doppler laser apparatus and optic-microcomputer analyser) or percentage of live spermatozoa (eosin-nigrosin staining) showed a higher repeatability for measurements of a same sample than for straws of a same ejaculate: the values were high (respectively greater than 0.78 and greater than 0.69) except for the optical analyser and staining. (2) Semen samples were collected from 80 stallions 5 times at 24-h intervals. The repeatability varied from 0.37 to 0.69 for gel-free volume, concentration, total sperm number, % of motile spermatozoa in raw and extended semen at collection or after storage. Therefore, to assess a stallion, 2-7 ejaculates, according to the characteristic being measured, are needed to get a repeatability of 0.80 for the mean value. (3) A study on 79 stallions serving mares over 4-152 oestrous cycles per year showed that 17 seasons are necessary to measure fertility based on the pregnancy rate per oestrous cycle with the same accuracy. If they served more than 25 cycles every year, 6 seasons only were required. In both cases, it was less than for % of live foals per season.
(1) 对活力的各种评估（主观判断、视频记录计数、多普勒激光仪器和光学 - 计算机分析仪）或活精子百分比（伊红 - 黑色素染色）显示，对同一样本的测量比对同一射精的细管样本具有更高的重复性：除了光学分析仪和染色外，这些值都很高（分别大于0.78和大于0.69）。(2) 从80匹种马中每隔24小时采集一次精液样本，共采集5次。对于无凝胶体积、浓度、总精子数、采集时或储存后原精液和稀释精液中活动精子的百分比，重复性在0.37至0.69之间。因此，为了评估一匹种马，根据所测量的特征，需要2至7次射精，以使平均值的重复性达到0.80。(3) 一项对79匹每年为母马配种4至152个发情周期的种马的研究表明，要以相同的准确性根据每个发情周期的妊娠率来测量生育力，需要17个季节。如果它们每年配种超过25个周期，则仅需要6个季节。在这两种情况下，所需时间都比每个季节活驹的百分比所需时间少。
