Crump A, Donaldson W L, Miller J, Kydd J H, Allen W R, Antczak D F
James A. Baker Institute for Animal Health, New York State College of Veterinary Medicine, Ithaca 14853.
J Reprod Fertil Suppl. 1987;35:379-88.
Antibodies to fetal major histocompatibility complex (MHC) antigens are routinely detected in the serum of pregnant mares some 2-4 weeks after formation of the endometrial cups at Day 36-38 after ovulation. Several experimental approaches were taken to determine whether paternal MHC antigens are expressed on horse placental tissues. First, absorption of anti-paternal MHC antisera with a large volume of endometrial cup cells removed antibody activity in only 2 of 4 experiments. Second, repeated immunization of horses with endometrial cup tissue recovered from a mare on Day 47 of pregnancy failed to induce the formation of anti-MHC antibodies. Third, a potent anti-MHC antiserum, raised in a pregnant mare which had previously received skin grafts from the MHC homozygous mating stallion, labelled chorionic girdle, but not normal allantochorion, when tested in an indirect immunoperoxidase labelling assay on tissues bearing the MHC antigens of the stallion. These results indicate that the rapidly dividing cells of the chorionic girdle, the progenitor tissue of the equine endometrial cups, express high levels of paternal MHC antigen, and may serve as the alloantigenic stimulus for cytotoxic antibody production by pregnant mares. Conversely, the mature, CG-secreting endometrial cup cells have a much reduced expression of paternal MHC antigen.
在排卵后第36 - 38天形成子宫内膜杯大约2 - 4周后，在怀孕母马的血清中通常能检测到针对胎儿主要组织相容性复合体（MHC）抗原的抗体。采用了几种实验方法来确定父本MHC抗原是否在马胎盘组织上表达。首先，用大量子宫内膜杯细胞吸收抗父本MHC抗血清，在4个实验中只有2个实验消除了抗体活性。其次，用从怀孕第47天的母马身上回收的子宫内膜杯组织反复免疫马，未能诱导抗MHC抗体的形成。第三，在一匹先前接受过来自MHC纯合交配种马皮肤移植的怀孕母马中产生的一种强效抗MHC抗血清，在对带有种马MHC抗原的组织进行间接免疫过氧化物酶标记试验时，标记了绒毛带，但未标记正常尿囊绒膜。这些结果表明，绒毛带（马子宫内膜杯的祖细胞组织）中快速分裂的细胞表达高水平的父本MHC抗原，并且可能作为怀孕母马产生细胞毒性抗体的同种异体抗原刺激物。相反，成熟的、分泌CG的子宫内膜杯细胞中父本MHC抗原的表达大大降低。
